Antibody:	anti-gamma H2AX
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Specimens were fixed in 1% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS21: ovary	Ambiguous		4B 13.5 dpc upper , 4B 13.5 dpc lower
TS21: germ cell of ovary	Absent		4B 13.5 dpc lower , 4B 13.5 dpc upper

Structure	Level	Pattern	Image	Note
TS22: ovary	Present	Regionally restricted	4B 14.5 dpc upper , 4B 14.5 dpc lower	Expression in an anterior to posterior pattern; germ cells became positive at the anterior end first.
TS22: germ cell of ovary	Present		4B 14.5 dpc upper , 4B 14.5 dpc lower	Staining in germ cells was first detected at 14.5 dpc.

Structure	Level	Pattern	Image	Note
TS23: ovary	Present	Regionally restricted	4B 15.5 dpc lower , 4B 15.5 dpc upper	Expression in germ cells throughout the gonad, in both cortex and medulla.
TS23: germ cell of ovary	Present	Widespread	4B 15.5 dpc lower , 4B 15.5 dpc upper	Expression in germ cells throughout the gonad, in both cortex and medulla.

Structure	Level	Pattern	Image	Note
TS21: testis	Ambiguous		5B 13.5 dpc
TS21: germ cell of testis	Absent		5B 13.5 dpc

Structure	Level	Pattern	Image	Note
TS22: testis	Present	Regionally restricted	5B 14.5 dpc
TS22: germ cell of testis	Present	Regionally restricted	5B 14.5 dpc	Authors state that 20-30 positive germ cells were found in the anterior junction between the gonad and mesonephros. Positive cells were located both inside and outside testis cord structures and in the mesonephros.
TS22: mesonephros	Present	Regionally restricted	5B 14.5 dpc

Structure	Level	Pattern	Image	Note
TS23: testis	Ambiguous		5B 15.5 dpc
TS23: germ cell of testis	Absent		5B 15.5 dpc