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Gene Expression Data
Assay Details
Assay
Reference: J:67011 Bain G, et al., E2A proteins are required for proper B cell development and initiation of immunoglobulin gene rearrangements. Cell. 1994 Dec 2;79(5):885-92
Assay type: RT-PCR
MGI Accession ID: MGI:2684392
Gene symbol: Rag1
Gene name: recombination activating 1
Probe: F3, R6
Assay notes: The RT-PCR products were hybridized with a gene specific primer.
Results Image: 5A RAG-1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
+/- E15.5 (a) TS23: liver Present 3 µg; total RNA either: 129P2/OlaHsd-Tcf3tm1Cmu or (involves: 129P2/OlaHsd * FVB/N) Tcf3tm1Cmu/Tcf3+Not Specified
+/- E15.5 (a) TS23: liver Present 3 µg; total RNA either: 129P2/OlaHsd-Tcf3tm1Cmu or (involves: 129P2/OlaHsd * FVB/N) Tcf3tm1Cmu/Tcf3+Not Specified
-/- E15.5 (a) TS23: liver Present (b) 3 µg; total RNA either: 129P2/OlaHsd-Tcf3tm1Cmu or (involves: 129P2/OlaHsd * FVB/N) Tcf3tm1Cmu/Tcf3tm1CmuNot Specified
-/- E15.5 (a) TS23: liver Present (b) 3 µg; total RNA either: 129P2/OlaHsd-Tcf3tm1Cmu or (involves: 129P2/OlaHsd * FVB/N) Tcf3tm1Cmu/Tcf3tm1CmuNot Specified
+/- E15.5 (a) TS23: liver Present 3 µg; total RNA either: 129P2/OlaHsd-Tcf3tm1Cmu or (involves: 129P2/OlaHsd * FVB/N) Tcf3tm1Cmu/Tcf3+Not Specified
+/+ E15.5 (a) TS23: liver Present 3 µg; total RNA Not Specified Not Specified
+/+ E15.5 (a) TS23: liver Present 3 µg; total RNA Not Specified Not Specified
+/- E15.5 (a) TS23: liver Present 3 µg; total RNA either: 129P2/OlaHsd-Tcf3tm1Cmu or (involves: 129P2/OlaHsd * FVB/N) Tcf3tm1Cmu/Tcf3+Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors stated that expression levels were dramatically reduced in the homozygotes relative to those seen in wild-type and heterozygotic embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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11/19/2024
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