Gli3 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:92194 Li Y, et al., Sonic hedgehog signaling regulates Gli3 processing, mesenchymal proliferation, and differentiation during mouse lung organogenesis. Dev Biol. 2004 Jun 1;270(1):214-31
Assay type:	Western blot
MGI Accession ID:	MGI:3526085
Gene symbol:	Gli3
Gene name:	GLI-Kruppel family member GLI3

Antibody:

anti-Gli3-N

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	190 kDa (b)	83 kDa (d)	Genetic Background	Mutant Allele(s)	Sex
1	E12.5 (a)	TS20: lung	Weak (c)	Strong (e)	involves: 129S1/Sv * 129X1/SvJ	Shh^tm1Chg/Shh^tm1Chg	Not Specified
2	E12.5 (a)	TS20: lung	Present	Present	Not Specified		Not Specified
3	E12.5 (a)	TS20: lung	Absent	Absent	involves: C3H/HeJ	Gli3^Xt-J/Gli3^Xt-J	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Prominent band corresponding to full-length Gli3-190.
(c) Authors report a reduction in Gli3-190 relative to wild-type.
(d) Authors report several 83K to 86K bands, corresponding to processed repressor form (Gli3R).
(e) Authors report higher levels of Gli3R relative to wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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