Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:99372
van der Zwaag B, et al., Identifying new candidate genes for hereditary facial paresis on chromosome 3q21-q22 by RNA in situ hybridization in mouse. Genomics. 2005 Jul;86(1):55-67
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:3605533
|
Gene symbol:
|
Abtb1
|
Gene name:
|
ankyrin repeat and BTB domain containing 1
|
Probe:
|
Abtb1 cDNA1
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
Assay notes:
|
After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde. The author divided the genes analyzed in this publication into 4 groups based on their expression patterns. The expression pattern of this gene was placed in group 3 (See Table 1).
|
|
Results
|
Specimen
2C:
embryonic day 16.5
(more )
Specimen
2C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: cerebral cortex |
Present |
Graded |
2C
|
Expression was detected in the intermediate zone of the neocortex in a gradient with highest expression in the lateral borders of the cerebral hemispheres and lower expression near midline.
|
TS24: cerebral cortex intermediate zone |
Strong |
|
2C
|
|
Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 16.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 18.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
|