Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS9: extraembryonic visceral endoderm	Strong		2B	Showed nuclear localization.
TS9: parietal endoderm	Strong		2B	Showed nuclear localization.
TS9: embryo ectoderm	Strong		2B	Showed nuclear and cytoplasmic localization in a small proportion of cells.
TS9: trophectoderm	Strong	Regionally restricted	2B	Expression was strong in the trophoblast. Showed nuclear and cytoplasmic localization in a small proportion of cells.
TS9: embryo	Present	Regionally restricted	2B	Expression was detected in cells in the epiblast.

Structure	Level	Pattern	Image	Note
TS11: trophectoderm	Strong	Regionally restricted	2C	Expression was detected in the trophoblast.
TS11: extraembryonic visceral endoderm	Strong		2C
TS11: node	Present		2C
TS11: primitive streak	Present		2C
TS11: embryo endoderm	Strong	Patchy	2C	Expression was strong in scattered cells in the embryonic endoderm.

Structure	Level	Pattern	Image	Note
TS11: extraembryonic visceral endoderm	Present		2D
TS11: neural ectoderm	Present	Regionally restricted	2D	Expression was detected in the anterior neurectoderm. Showed both cytoplasmic and nuclear localization.
TS11: primitive streak	Present	Regionally restricted	2D	In the primitive streak, cells in the ectoderm rapidly downregulate reporter expression after ingression to the adjacent mesodermal layer. Showed both cytoplasmic and nuclear localization.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present		3A
TS14: optic vesicle	Present		3A
TS14: somite	Present	Non-Uniform	3A	Expression was restricted to the anterior half of the condensing and most recently formed somites while it seemed weaker and uniformly distributed in more differentiated somites.

Structure	Level	Pattern	Image	Note
TS14: primitive streak	Present	Regionally restricted	3B	Expression was detected in the ectodermal layer of the primitive streak.
TS14: somite	Present	Non-Uniform	3B	Expression was upregulated in somites emerging from the presomitic mesoderm.
TS14: gut	Strong	Regionally restricted	3B	Expression was intense in a subset of cells in the the gut.

Structure	Level	Pattern	Image	Note
TS14: somite	Present	Regionally restricted	3C	Expression is detected in the anterior compartment of the nascent somite (s0) and three most recently formed somites (sI to sIII).
TS14: somite 01	Present	Regionally restricted	3C	Expression is detected in the anterior compartment of the nascent somite (s0) and three most recently formed somites (sI to sIII).
TS14: somite 02	Present	Regionally restricted	3C	Expression is detected in the anterior compartment of the nascent somite (s0) and three most recently formed somites (sI to sIII).
TS14: somite 03	Present	Regionally restricted	3C	Expression is detected in the anterior compartment of the nascent somite (s0) and three most recently formed somites (sI to sIII).
TS14: somite 04	Present	Uniform	3C	Expression in more mature somites (sIV) was lower and more evenly distributed throughout the somite compared to the most recently formed somites.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		3D
TS20: limb	Present		3D
TS20: nervous system	Present		3D
TS20: pinna	Present		3D
TS20: tail somite	Present		3D
TS20: skeletal muscle	Present		3D , 3E
TS20: limb interdigital region	Present	Regionally restricted	3E	Expression was detected in cells surrounding the digits.

Structure	Level	Pattern	Image	Note
TS20: tail	Strong	Regionally restricted	3F	Expression was high in the chordoneural hinge.
TS20: tail somite	Present		3F

Structure	Level	Pattern	Image	Note
TS23: heart	Strong		3G
TS23: intercostal muscle	Strong		3G
TS23: liver	Present		3G , 3K

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Regionally restricted	3I , 3H	Expression was detected in the ventricular zone of the cortex and at higher levels in more differentiated progeny. B-Gal localization was mainly cytoplasmic in the neural tissue.
TS23: cerebral cortex ventricular layer	Present	Regionally restricted	3I , 3H	Expression was detected in the ventricular zone of the cortex and at higher levels in more differentiated progeny. B-Gal localization was mainly cytoplasmic in the neural tissue.

Structure	Level	Pattern	Image	Note
TS23: skeletal muscle	Present		3J	B-Gal localization was mainly nuclear in the musculature.

Structure	Level	Pattern	Image	Note
TS23: skin	Strong	Regionally restricted		Expression was strong in deposits of brown fat.
TS23: intestine epithelium	Strong
TS23: submandibular gland primordium	Strong
TS23: metanephros	Strong	Regionally restricted		Expression was strong in kidney epithelium.
TS23: testis	Strong	Regionally restricted		Expression was strong in testis epithelium.