Probe:	Zimp7 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Embryos were re-fixed with 0.2% glutaraldehyde/ 4% PFA prior to hybridization.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		3A
TS11: neural ectoderm	Present	Regionally restricted	3A	Expression is in an anterior region.
TS11: embryo	Weak		3A	Expression is weak in the epiblast.
TS11: chorion	Weak		3A
TS11: embryo mesoderm	Weak		3A

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Present	Regionally restricted	3B	Expression is in the anterior mesenchyme extending up to the second anterior somite.
TS13: somite 01	Present	Regionally restricted	3B	Expression is in the anterior mesenchyme extending up to the second anterior somite.
TS13: branchial arch	Present		3B
TS13: foregut diverticulum	Present		3B
TS13: future hindbrain	Present		3B
TS13: future midbrain	Present		3B
TS13: notochord	Present		3B

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Present	Regionally restricted	3C	Expression is in the anterior mesenchyme extending up to the second anterior somite.
TS13: somite 01	Present	Regionally restricted	3C	Expression is in the anterior mesenchyme extending up to the second anterior somite.
TS13: branchial arch	Present		3C
TS13: foregut diverticulum	Present		3C
TS13: future hindbrain	Present		3C
TS13: future midbrain	Present		3C
TS13: notochord	Present		3C
TS13: future prosencephalon	Absent		3C

Structure	Level	Pattern	Image	Note
TS13: gut endoderm	Present		3D
TS13: neural ectoderm	Present		3D

Structure	Level	Pattern	Image	Note
TS14: embryo	Strong	Regionally restricted	3E	There is strong expression in the anterior embryo, absent in the caudal region.
TS14: branchial arch	Strong		3E
TS14: future brain	Strong		3E
TS14: head mesenchyme	Strong		3E
TS14: otic pit	Strong		3E
TS14: primitive ventricle	Absent		3E
TS14: somite	Absent		3E

Structure	Level	Pattern	Image	Note
TS14: embryo	Strong	Regionally restricted	3F	There is strong ubiquitous expression from the top of the head up to the heart level and is absent in the caudal region.
TS14: branchial arch	Strong		3F
TS14: future brain	Strong		3F
TS14: head mesenchyme	Strong		3F
TS14: otic pit	Strong		3F
TS14: primitive ventricle	Absent		3F
TS14: somite	Absent		3F

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Regionally restricted	3G	Expression is anterior.
TS15: forelimb bud	Present		3G
TS15: hindlimb ridge	Present		3G

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present		4A
TS17: 1st branchial arch maxillary component	Present		4A
TS17: head mesenchyme	Present		4A
TS17: optic cup	Present		4A
TS17: otocyst	Present		4A
TS17: trunk mesenchyme	Present		4A
TS17: forebrain	Present	Regionally restricted	4A	Expression is in the ventral forebrain.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present		4B
TS17: 1st branchial arch maxillary component	Present		4B
TS17: head mesenchyme	Present		4B
TS17: lung	Present		4B
TS17: optic cup	Present		4B
TS17: otocyst	Present		4B
TS17: stomach	Present		4B
TS17: trunk mesenchyme	Present		4B
TS17: forebrain	Present	Regionally restricted	4B	Expression is in the ventral forebrain.
TS17: gut	Present	Regionally restricted	4B	Expression is in the intestine.

Structure	Level	Pattern	Image	Note
TS19: brain	Present		4D
TS19: limb	Present		4D
TS19: neural tube	Present		4D

Structure	Level	Pattern	Image	Note
TS19: brain	Present		4E	There are sharp boundaries around the telencephalic and mesencephalic vesicles.
TS19: limb	Present		4E
TS19: neural tube	Present		4E

Structure	Level	Pattern	Image	Note
TS20: brain	Present
TS20: limb	Present
TS20: spinal cord	Present