Probe:	frataxin probe 1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Two additional probes resulted in weaker signals but generated essentially identical patterns of hybridization; see MGI:1099211 and MGI:1099209 for probe information.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Present	Regionally restricted	2b	Expression was detected at the junction of the pons and the medulla oblongata.
TS20: pons	Present	Regionally restricted	2b	Expression was detected at the junction of the pons and the medulla oblongata.
TS20: ganglionic eminence	Weak		2b
TS20: head mesenchyme	Weak	Regionally restricted	2b	Expression was detected in facial mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Strong		2c
TS24: forebrain	Strong		2c
TS24: heart atrium	Strong		2c
TS24: lower jaw incisor epithelium	Strong		2c
TS24: spinal cord	Strong		2c
TS24: hindbrain	Present		2c
TS24: midbrain	Present		2c

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex ventricular layer	Strong		3a , 4a
TS24: cortical plate	Strong		3a , 4a
TS24: cerebral cortex intermediate zone	Trace		4a , 3a
TS24: ganglionic eminence	Very strong		4b , 3a
TS24: diencephalon	Weak		3a
TS24: olfactory lobe	Present	Regionally restricted	3a	Expression was detected in the extension of the anterior horn of the lateral ventricle into the olfactory lobe.
TS24: skin	Present		3a

Structure	Level	Pattern	Image	Note
TS24: medulla oblongata	Present		4c

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Present	Non-Uniform	5c , 5a	Expression was detected throughout the spinal cord with intense staining observed in the thoracolumbar level.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present	Regionally restricted	6a	Expression was detected in large round cells in the dorsal root ganglia.
TS22: rib cartilage condensation	Present		6a
TS22: thoracic vertebral cartilage condensation	Present		6a

Structure	Level	Pattern	Image	Note
TS22: vertebral cartilage condensation	Strong		6b
TS22: intervertebral disc	Absent		6b
TS22: spinal cord	Present		6b

Structure	Level	Pattern	Image	Note
TS24: heart atrium	Strong	Regionally restricted	7a	Expression was robust in the wall of the atrium.
TS24: atrioventricular endocardial cushion	Present		7a
TS24: inferior vena cava	Present	Regionally restricted	7a	Expression was detected in the wall of the inferior vena cava entering the atrium.

Structure	Level	Pattern	Image	Note
TS24: lower jaw incisor enamel organ	Strong		7b
TS24: lower jaw incisor mesenchyme	Present		7b

Structure	Level	Pattern	Image	Note
TS16: central nervous system	Trace	Regionally restricted		Expression was very weak in the neuroepithelium.