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Gene Expression Data
Assay Details
Assay
Reference: J:140465 Guo G, et al., Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. Dev Cell. 2010 Apr 20;18(4):675-85
Assay type: RT-PCR
MGI Accession ID: MGI:3816592
Gene symbol: Hltf
Gene name: helicase-like transcription factor
Probe: Mm00447302_m1
Assay notes: These results were obtained using TaqMan real time PCR. For each embryonic stage, 150 embryos were harvested and pooled together. The inner cell mass tissue was isolated using immunosurgery.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
Metaphase II P TS28: oocyte Moderate Not Specified; total RNA (C57BL/6 x CBA)F1 Female
2-cell embryo E1.5 TS2: 2-cell stage conceptus Trace Not Specified; total RNA involves: C57BL/6 * CBA * CD-1 Not Specified
4-cell embryo E2.0 TS3: 4-cell stage conceptus Trace Not Specified; total RNA involves: C57BL/6 * CBA * CD-1 Not Specified
8-cell embryo E2.5 TS3: 8-cell stage conceptus Weak Not Specified; total RNA involves: C57BL/6 * CBA * CD-1 Not Specified
Morula E3.0 TS3: morula-stage conceptus Weak Not Specified; total RNA involves: C57BL/6 * CBA * CD-1 Not Specified
E3.5 blastocyst E3.5 TS4: blastocyst-stage conceptus Moderate Not Specified; total RNA involves: C57BL/6 * CBA * CD-1 Not Specified
E4.5 blastocyst E4.5 TS5: blastocyst-stage conceptus Moderate Not Specified; total RNA involves: C57BL/6 * CBA * CD-1 Not Specified
E4.5 inner cell mass E4.5 TS5: inner cell mass Weak Not Specified; total RNA involves: C57BL/6 * CBA * CD-1 Not Specified
Notes:
(a) Ct values of 35 or higher were interpreted as absent; 33-34=trace; 31-32=weak; 29-30=moderate; 27-28=strong; and 26 or lower=very strong.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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11/19/2024
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