Probe:	UI-M-BH2.1-aps-f-08-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Moderate	Non-Uniform	Rnf152-Shh E11.5_sagittal	There is moderate staining in the MZ of the prethalamus. Staining is either absent or ubiquitous in the MZ of the dorsal thalamus, dorsal & ventral POAs, the PVH, VMH & ARH nuclei, the AVH, anterior & posterior IDs, premam, mam & supramam zones & the TT.
TS19: diencephalon lateral wall ventricular layer	Ambiguous		Rnf152-Shh E11.5_sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: telencephalon mantle layer	Ambiguous		Rnf152-Shh E11.5_sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer	Ambiguous		Rnf152-Shh E11.5_sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Rnf152-Shh E12.5_sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Ambiguous		Rnf152-Shh E12.5_sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Rnf152-Shh E12.5_sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Weak	Non-Uniform	Rnf152-Shh E12.5_sagittal	There is weak staining in the mantle zone of the supramam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and mam zones and the TT.
TS20: diencephalon ventricular layer	Ambiguous		Rnf152-Shh E12.5_sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence	Ambiguous		Rnf152-Shh E12.5_sagittal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer	Strong	Non-Uniform	Rnf152-Shh E12.5_sagittal	There is strong staining in the MZ of the AVH, moderate staining in the PVH nucleus and weak staining in the ventral POA. Staining is either absent or ubiquitous in the MZ of the dorsal POA, the VMH and ARH nuclei and anterior and posterior IDs.
TS20: hypothalamus ventricular layer	Ambiguous		Rnf152-Shh E12.5_sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer	Strong	Non-Uniform	Rnf152-Shh E12.5_sagittal	There is strong staining in the mantle zone of the dorsal thalamus and weak staining in the prethalamus.
TS20: thalamus ventricular layer	Ambiguous		Rnf152-Shh E12.5_sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		Rnf152-Shh E14.5_sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Ambiguous		Rnf152-Shh E14.5_sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		Rnf152-Shh E14.5_sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Weak	Non-Uniform	Rnf152-Shh E14.5_sagittal	There is weak staining in the mantle zone of the supramam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and mam zones and the TT.
TS22: diencephalon ventricular layer	Ambiguous		Rnf152-Shh E14.5_sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence	Ambiguous		Rnf152-Shh E14.5_sagittal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer	Weak	Non-Uniform	Rnf152-Shh E14.5_sagittal	There is weak staining in the mantle zone of the PVH and VMH nuclei. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the ARH nucleus, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer	Ambiguous		Rnf152-Shh E14.5_sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer	Strong	Non-Uniform	Rnf152-Shh E14.5_sagittal	There is strong staining in the mantle zone of the dorsal thalamus and weak staining in the prethalamus.
TS22: thalamus ventricular layer	Ambiguous		Rnf152-Shh E14.5_sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Ambiguous		Rnf152-Shh E16.5_sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Ambiguous		Rnf152-Shh E16.5_sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Ambiguous		Rnf152-Shh E16.5_sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Moderate	Non-Uniform	Rnf152-Shh E16.5_sagittal	There is moderate staining in the mantle zone of the mam zone and weak staining in the supramam zone. Staining is either absent or ubiquitous in the mantle zone of the premam zone.
TS24: diencephalon ventricular layer	Ambiguous		Rnf152-Shh E16.5_sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence	Ambiguous		Rnf152-Shh E16.5_sagittal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer	Weak	Non-Uniform	Rnf152-Shh E16.5_sagittal	There is weak staining in the mantle zone of the dorsal and ventral POAs, the AVH, the DAH and the VMH nucleus. Staining is either absent or ubiquitous in the mantle zone of the PVH, SCH, DMH and ARH nuclei and the PH.
TS24: hypothalamus ventricular layer	Weak	Non-Uniform	Rnf152-Shh E16.5_sagittal	There is weak staining in the ventricular zone of the DMH and VMH nuclei. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer	Moderate	Non-Uniform	Rnf152-Shh E16.5_sagittal	There is moderate staining in the mantle zone of the dorsal thalamus and weak staining in the prethalamus.
TS24: thalamus ventricular layer	Ambiguous		Rnf152-Shh E16.5_sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.