Kat6a RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:174767 Tang F, et al., Deterministic and stochastic allele specific gene expression in single mouse blastomeres. PLoS One. 2011;6(6):e21208
Assay type:	RT-PCR
MGI Accession ID:	MGI:5289322
Gene symbol:	Kat6a
Gene name:	K(lysine) acetyltransferase 6A

Probe:	Mm03053529_s1
Assay notes:	These results were reported in Table S6. They were obtained using TaqMan real time PCR using individual blastomeres from either 2- or 4-cell embryos. In the Supplementary Materials and Methods, the authors reported that a gene was detected when the real-time PCR reaction had a Ct value of <32. Thus, we have interpreted Ct values of <=31.5 as present, >=32.5 as absent, and 31.6-32.4 as ambiguous.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
2-cell-A1-1	E1.5	TS2: 2-cell stage embryo	Absent (a)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 1 from embryo A.
2-cell-A1-2	E1.5	TS2: 2-cell stage embryo	Absent (b)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 1 from embryo A.
2-cell-A2-1	E1.5	TS2: 2-cell stage embryo	Ambiguous (c)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 2 from embryo A.
2-cell-A2-2	E1.5	TS2: 2-cell stage embryo	Present (d)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 2 from embryo A.
2-cell-B1-1	E1.5	TS2: 2-cell stage embryo	Absent (e)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 1 from embryo B.
2-cell-B1-2	E1.5	TS2: 2-cell stage embryo	Absent (f)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 1 from embryo B.
2-cell-B2-1	E1.5	TS2: 2-cell stage embryo	Absent (g)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 2 from embryo B.
2-cell-B2-2	E1.5	TS2: 2-cell stage embryo	Ambiguous (h)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 2 from embryo B.
2-cell-C1-1	E1.5	TS2: 2-cell stage embryo	Present (i)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 1 from embryo C.
2-cell-C1-2	E1.5	TS2: 2-cell stage embryo	Present (j)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 1 from embryo C.
2-cell-C2-1	E1.5	TS2: 2-cell stage embryo	Present (k)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 2 from embryo C.
2-cell-C2-2	E1.5	TS2: 2-cell stage embryo	Present (l)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 2 from embryo C.
4-cell-A1-1	E2.0	TS3: 4-cell stage embryo	Present (m)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 1 from embryo A.
4-cell-A1-2	E2.0	TS3: 4-cell stage embryo	Present (n)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 1 from embryo A.
4-cell-A2-1	E2.0	TS3: 4-cell stage embryo	Absent (a)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 2 from embryo A.
4-cell-A2-2	E2.0	TS3: 4-cell stage embryo	Absent (a)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 2 from embryo A.
4-cell-A3-1	E2.0	TS3: 4-cell stage embryo	Present (o)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 3 from embryo A.
4-cell-A3-2	E2.0	TS3: 4-cell stage embryo	Present (p)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 3 from embryo A.
4-cell-A4-1	E2.0	TS3: 4-cell stage embryo	Present (q)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 4 from embryo A.
4-cell-A4-2	E2.0	TS3: 4-cell stage embryo	Present (r)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 4 from embryo A.
4-cell-B1-1	E2.0	TS3: 4-cell stage embryo	Present (s)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 1 from embryo B.
4-cell-B1-2	E2.0	TS3: 4-cell stage embryo	Present (t)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 1 from embryo B.
4-cell-B2-1	E2.0	TS3: 4-cell stage embryo	Present (u)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 2 from embryo B.
4-cell-B2-2	E2.0	TS3: 4-cell stage embryo	Present (v)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 2 from embryo B.
4-cell-B3-1	E2.0	TS3: 4-cell stage embryo	Absent (w)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 3 from embryo B.
4-cell-B3-2	E2.0	TS3: 4-cell stage embryo	Ambiguous (x)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 3 from embryo B.
4-cell-B4-1	E2.0	TS3: 4-cell stage embryo	Absent (y)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 4 from embryo B.
4-cell-B4-2	E2.0	TS3: 4-cell stage embryo	Absent (z)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 4 from embryo B.
4-cell-C1-1	E2.0	TS3: 4-cell stage embryo	Absent (a)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 1 from embryo C.
4-cell-C1-2	E2.0	TS3: 4-cell stage embryo	Absent (a)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 1 from embryo C.
4-cell-C2-1	E2.0	TS3: 4-cell stage embryo	Present (A)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 2 from embryo C.
4-cell-C2-2	E2.0	TS3: 4-cell stage embryo	Present (B)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 2 from embryo C.
4-cell-C3-1	E2.0	TS3: 4-cell stage embryo	Present (C)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 3 from embryo C.
4-cell-C3-2	E2.0	TS3: 4-cell stage embryo	Present (D)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 3 from embryo C.
4-cell-C4-1	E2.0	TS3: 4-cell stage embryo	Absent (E)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 4 from embryo C.
4-cell-C4-2	E2.0	TS3: 4-cell stage embryo	Ambiguous (F)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 4 from embryo C.

Notes:
(a) The Ct value of this reaction was 40.
(b) The Ct value of this reaction was 38.97.
(c) The Ct value of this reaction was 31.68.
(d) The Ct value of this reaction was 31.28.
(e) The Ct value of this reaction was 35.49.
(f) The Ct value of this reaction was 36.61.
(g) The Ct value of this reaction was 32.69.
(h) The Ct value of this reaction was 32.16.
(i) The Ct value of this reaction was 30.08.
(j) The Ct value of this reaction was 30.03.
(k) The Ct value of this reaction was 30.44.
(l) The Ct value of this reaction was 30.64.
(m) The Ct value of this reaction was 30.99.
(n) The Ct value of this reaction was 30.56.
(o) The Ct value of this reaction was 30.45.
(p) The Ct value of this reaction was 30.48.
(q) The Ct value of this reaction was 30.82.
(r) The Ct value of this reaction was 30.89.
(s) The Ct value of this reaction was 31.13.
(t) The Ct value of this reaction was 30.97.
(u) The Ct value of this reaction was 29.21.
(v) The Ct value of this reaction was 29.12.
(w) The Ct value of this reaction was 32.54.
(x) The Ct value of this reaction was 31.94.
(y) The Ct value of this reaction was 32.93.
(z) The Ct value of this reaction was 32.98.
(A) The Ct value of this reaction was 30.98.
(B) The Ct value of this reaction was 30.8.
(C) The Ct value of this reaction was 29.03.
(D) The Ct value of this reaction was 28.75.
(E) The Ct value of this reaction was 32.58.
(F) The Ct value of this reaction was 32.47.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 11/19/2024 MGI 6.24