Kdm5b RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:174767 Tang F, et al., Deterministic and stochastic allele specific gene expression in single mouse blastomeres. PLoS One. 2011;6(6):e21208
Assay type:	RT-PCR
MGI Accession ID:	MGI:5289649
Gene symbol:	Kdm5b
Gene name:	lysine demethylase 5B

Probe:	Mm03053411_s1
Assay notes:	These results were reported in Table S6. They were obtained using TaqMan real time PCR using individual blastomeres from either 2- or 4-cell embryos. In the Supplementary Materials and Methods, the authors reported that a gene was detected when the real-time PCR reaction had a Ct value of <32. Thus, we have interpreted Ct values of <=31.5 as present, >=32.5 as absent, and 31.6-32.4 as ambiguous.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
2-cell-A1-1	E1.5	TS2: 2-cell stage embryo	Present (a)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 1 from embryo A.
2-cell-A1-2	E1.5	TS2: 2-cell stage embryo	Present (b)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 1 from embryo A.
2-cell-A2-1	E1.5	TS2: 2-cell stage embryo	Present (c)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 2 from embryo A.
2-cell-A2-2	E1.5	TS2: 2-cell stage embryo	Present (d)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 2 from embryo A.
2-cell-B1-1	E1.5	TS2: 2-cell stage embryo	Present (e)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 1 from embryo B.
2-cell-B1-2	E1.5	TS2: 2-cell stage embryo	Present (f)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 1 from embryo B.
2-cell-B2-1	E1.5	TS2: 2-cell stage embryo	Present (g)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 2 from embryo B.
2-cell-B2-2	E1.5	TS2: 2-cell stage embryo	Present (h)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 2 from embryo B.
2-cell-C1-1	E1.5	TS2: 2-cell stage embryo	Present (i)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 1 from embryo C.
2-cell-C1-2	E1.5	TS2: 2-cell stage embryo	Present (j)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 1 from embryo C.
2-cell-C2-1	E1.5	TS2: 2-cell stage embryo	Present (k)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 2 from embryo C.
2-cell-C2-2	E1.5	TS2: 2-cell stage embryo	Present (l)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 2 from embryo C.
4-cell-A1-1	E2.0	TS3: 4-cell stage embryo	Present (m)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 1 from embryo A.
4-cell-A1-2	E2.0	TS3: 4-cell stage embryo	Present (n)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 1 from embryo A.
4-cell-A2-1	E2.0	TS3: 4-cell stage embryo	Present (o)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 2 from embryo A.
4-cell-A2-2	E2.0	TS3: 4-cell stage embryo	Present (p)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 2 from embryo A.
4-cell-A3-1	E2.0	TS3: 4-cell stage embryo	Present (q)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 3 from embryo A.
4-cell-A3-2	E2.0	TS3: 4-cell stage embryo	Present (r)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 3 from embryo A.
4-cell-A4-1	E2.0	TS3: 4-cell stage embryo	Present (s)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 4 from embryo A.
4-cell-A4-2	E2.0	TS3: 4-cell stage embryo	Present (t)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 4 from embryo A.
4-cell-B1-1	E2.0	TS3: 4-cell stage embryo	Ambiguous (u)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 1 from embryo B.
4-cell-B1-2	E2.0	TS3: 4-cell stage embryo	Present (v)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 1 from embryo B.
4-cell-B2-1	E2.0	TS3: 4-cell stage embryo	Present (p)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 2 from embryo B.
4-cell-B2-2	E2.0	TS3: 4-cell stage embryo	Present (w)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 2 from embryo B.
4-cell-B3-1	E2.0	TS3: 4-cell stage embryo	Absent (x)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 3 from embryo B.
4-cell-B3-2	E2.0	TS3: 4-cell stage embryo	Absent (x)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 3 from embryo B.
4-cell-B4-1	E2.0	TS3: 4-cell stage embryo	Present (y)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 4 from embryo B.
4-cell-B4-2	E2.0	TS3: 4-cell stage embryo	Present (z)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 4 from embryo B.
4-cell-C1-1	E2.0	TS3: 4-cell stage embryo	Present (A)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 1 from embryo C.
4-cell-C1-2	E2.0	TS3: 4-cell stage embryo	Present (B)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 1 from embryo C.
4-cell-C2-1	E2.0	TS3: 4-cell stage embryo	Present (C)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 2 from embryo C.
4-cell-C2-2	E2.0	TS3: 4-cell stage embryo	Present (D)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 2 from embryo C.
4-cell-C3-1	E2.0	TS3: 4-cell stage embryo	Present (E)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 3 from embryo C.
4-cell-C3-2	E2.0	TS3: 4-cell stage embryo	Present (F)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 3 from embryo C.
4-cell-C4-1	E2.0	TS3: 4-cell stage embryo	Present (G)	Not Specified; total RNA	MF1		Not Specified	This is the first replicate using blastomere 4 from embryo C.
4-cell-C4-2	E2.0	TS3: 4-cell stage embryo	Present (H)	Not Specified; total RNA	MF1		Not Specified	This is the second replicate using blastomere 4 from embryo C.

Notes:
(a) The Ct value of this reaction was 30.32.
(b) The Ct value of this reaction was 30.31.
(c) The Ct value of this reaction was 29.62.
(d) The Ct value of this reaction was 29.51.
(e) The Ct value of this reaction was 29.12.
(f) The Ct value of this reaction was 29.17.
(g) The Ct value of this reaction was 29.56.
(h) The Ct value of this reaction was 29.35.
(i) The Ct value of this reaction was 30.15.
(j) The Ct value of this reaction was 29.99.
(k) The Ct value of this reaction was 28.54.
(l) The Ct value of this reaction was 28.55.
(m) The Ct value of this reaction was 30.3.
(n) The Ct value of this reaction was 30.09.
(o) The Ct value of this reaction was 30.58.
(p) The Ct value of this reaction was 30.48.
(q) The Ct value of this reaction was 30.97.
(r) The Ct value of this reaction was 31.03.
(s) The Ct value of this reaction was 29.6.
(t) The Ct value of this reaction was 29.85.
(u) The Ct value of this reaction was 31.65.
(v) The Ct value of this reaction was 31.29.
(w) The Ct value of this reaction was 30.41.
(x) The Ct value of this reaction was 40.
(y) The Ct value of this reaction was 30.39.
(z) The Ct value of this reaction was 30.35.
(A) The Ct value of this reaction was 29.66.
(B) The Ct value of this reaction was 29.63.
(C) The Ct value of this reaction was 28.09.
(D) The Ct value of this reaction was 28.04.
(E) The Ct value of this reaction was 29.16.
(F) The Ct value of this reaction was 29.19.
(G) The Ct value of this reaction was 31.53.
(H) The Ct value of this reaction was 31.31.

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