Antibody:	anti-Atxn3
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: skeletal muscle	Strong		6A

Structure	Level	Pattern	Image	Note
TS28: skeletal muscle	Strong		6B

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Strong		6C

Structure	Level	Pattern	Image	Note
TS28: smooth muscle tissue	Strong		6D

Structure	Level	Pattern	Image	Note
TS28: pancreas	Present	Ubiquitous	6E
TS28: exocrine pancreas	Present		6E
TS28: islets of Langerhans	Present		6E

Structure	Level	Pattern	Image	Note
TS28: spleen red pulp	Present		6G
TS28: spleen white pulp	Present		6G

Structure	Cell Type	Level	Pattern	Image	Note
TS28: testis		Present	Widespread	6H
TS28: Sertoli cell	Sertoli cell	Absent		6H
TS28: spermatid		Present		6H
TS28: spermatocyte		Present		6H
TS28: spermatogonium		Present		6H
TS28: spermatozoon		Present		6H
TS28: tunica albuginea of testis		Strong		6H
TS28: interstitium of the testis		Present		6H

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Widespread	6I

Structure	Level	Pattern	Image	Note
TS28: liver	Present		6J	Expression is present in hepatocytes.

Structure	Level	Pattern	Image	Note
TS28: spermatozoon	Strong		6K	Expression is present in the flagellum.

Structure	Level	Pattern	Image	Note
TS28: central nervous system	Present	Ubiquitous	7A	Expression is present in the cytoplasm of both neurons and glia.
TS28: brainstem	Present		7A
TS28: cerebellum	Present		7A

Structure	Level	Pattern	Image	Note
TS28: 4th ventricle ependyma	Present		7C

Structure	Level	Pattern	Image	Note
TS28: 4th ventricle ependyma	Strong		7D	Expression is present in the cells of the ependyma, including in their cilia.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		7E
TS28: cerebellum granule cell layer	Present		7E
TS28: cerebellum molecular layer	Present		7E

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		7F

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Widespread	7G	Expression is present in all layers of the cerebral cortex. Myelinated fibers also stained, although that was less pronounced.
TS28: cortical layer I	Present		7G
TS28: cortical layer II	Present		7G
TS28: cortical layer III	Present		7G
TS28: cortical layer IV	Present		7G
TS28: cortical layer V	Present		7G
TS28: cortical layer VI	Present		7G

Structure	Level	Pattern	Image	Note
TS28: brainstem	Present		7H	Expression is present in neurons.

Structure	Level	Pattern	Image	Note
TS28: substantia nigra pars compacta	Present		7I	Expression is present in neurons.
TS28: substantia nigra pars reticulata	Present		7I	Expression is present in neurons.

Structure	Level	Pattern	Image	Note
TS28: 3rd ventricle ependyma	Strong			Expression is present in the cells of the ependyma, including in their cilia.
TS28: lateral ventricle ependyma	Strong			Expression is present in the cells of the ependyma, including in their cilia.
TS28: anterior commissure	Present			Staining is less intense in fiber areas (but it is present).
TS28: corpus callosum	Present			Staining is less intense in fiber areas (but it is present).

Structure	Level	Pattern	Image	Note
TS19: embryo	Present		8A	Expression was observed in several tissues.
TS19: central nervous system	Present		8A

Structure	Level	Pattern	Image	Note
TS20: embryo	Present		8B	Expression was observed in several tissues.
TS20: central nervous system	Present		8B

Structure	Level	Pattern	Image	Note
TS21: embryo	Present		8C	Expression was observed in several tissues.
TS21: central nervous system	Present		8C

Structure	Level	Pattern	Image	Note
TS22: embryo	Present		8D	Expression was observed in several tissues.
TS22: central nervous system	Present		8D

Structure	Level	Pattern	Image	Note
TS23: embryo	Present		8E	Expression was observed in several tissues.
TS23: central nervous system	Present		8E

Structure	Level	Pattern	Image	Note
TS24: central nervous system	Present		8F
TS24: cardiac muscle tissue	Present		8F
TS24: skeletal muscle	Present		8F
TS24: smooth muscle tissue	Present		8F
TS24: oral epithelium	Present		8F	Expression is present in the nonkeratinizing stratified squamous epithelium.
TS24: nasopharynx epithelium	Present		8F	Expression is present in the nonkeratinizing stratified squamous epithelium.
TS24: intestine epithelium	Present		8F	Expression is present in the apical portion of the enterocytes.
TS24: adrenal gland	Present		8F
TS24: pancreas	Present		8F
TS24: sublingual gland	Present		8F
TS24: submandibular gland	Present		8F
TS24: cochlea epithelium	Strong		8F , 8H
TS24: olfactory epithelium	Strong		8F , 8I
TS24: metanephros	Present		8J , 8F
TS24: lung	Present		8K , 8F
TS24: stomach	Present		8F , 8L
TS24: thymus	Present		8M , 8F	Expression is present in both the medulla and cortex.
TS24: cartilage	Present	Non-Uniform	8N , 8F	Expression is present in ossification areas--the periosteum, osteoprogenitor cells; osteoblasts appeared to exhibit no staining.