Probe:	PGP9.5 probe1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Weak	Widespread		Low level of expression throughout the neural epithelium along the length of the undifferentiated neural tube

Structure	Level	Pattern	Image	Note
TS15: future brain	Moderate	Widespread	1A	Moderately abundant expression throughout the cross-sectional area of the neural tube and ganglia primordia.
TS15: neural tube	Moderate	Widespread	1A	Moderately abundant expression throughout the cross-sectional area of the neural tube and ganglia primordia.

Structure	Level	Pattern	Image	Note
TS17: brain	Strong		1C

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Strong	Regionally restricted	2A	High levels of expression in ventral motor areas of hindbrain.
TS19: cranial ganglion	Strong		2A

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Strong	Regionally restricted	2D	High levels of expression in ventral horns of the spinal cord.
TS19: dorsal root ganglion	Strong		2D

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Strong	Widespread	3A	Increased overall expression throughout the neural tube.
TS20: hindbrain	Strong	Regionally restricted	3A	Highest expression in ventral motor regions of the hindbrain.
TS20: cranial ganglion	Strong		3A
TS20: dorsal root ganglion	Strong		3A
TS20: spinal cord	Strong		3A
TS20: peripheral nervous system	Present	Regionally restricted	3A
TS20: gonad	Very strong		3A

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	3C	In the brain, expression is highest in the more differentiated lateral cells; lower level of expression in the proliferating medial cells.
TS20: olfactory epithelium	Very strong	Regionally restricted	3C	Authors report exceptionally abundant expression in sensory neurons of the nasal epithelia.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Strong	Widespread	3E	Increased overall abundance of expression throughout the neural tube.
TS20: dorsal root ganglion	Strong		3E

Structure	Level	Pattern	Image	Note
TS23: brain	Strong		4A	Authors report abundant expression in all of the sites mentioned for the previous stages.
TS23: spinal cord	Strong		4A	Authors report abundant expression in all of the sites mentioned for the previous stages.
TS23: neural retina	Strong		4A

Structure	Level	Pattern	Image	Note
TS23: adrenal medulla	Strong	Regionally restricted	4B	Abundant expression in in neuroendocrine cells of the adrenal medulla.
TS23: enteric nervous system	Present	Regionally restricted	4B	Expression in nerve plexi of the gut.

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Present		4C
TS23: superior cervical ganglion	Present		4C

Structure	Level	Pattern	Image	Note
TS27: brain	Strong	Regionally restricted	5A
TS27: cerebellar cortex	Very strong	Regionally restricted	5A	Most abundant expression in outer layers of the cerebral and cerebellar cortex.
TS27: cerebral cortex	Very strong	Regionally restricted	5A	Most abundant expression in outer layers of the cerebral and cerebellar cortex.
TS27: thalamus	Strong		5A
TS27: tectum	Strong	Regionally restricted	5A	Abundant expression in colliculi.
TS27: brain ependyma	Absent		5A