Antibody:	anti-PGP9.5
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Some sections, indicated as unmasked, were microwave boiled in citrate buffer prior to application of the primary antibody.

Structure	Level	Pattern	Image	Note
TS15: embryo	Trace			Authors state that expression is barely detectable.

Structure	Level	Pattern	Image	Note
TS17: brain	Present		1D	Expression throughout the neural tube and in all sensory ganglia.
TS17: cranial ganglion	Present		1D	Expression throughout the neural tube and in all sensory ganglia.

Structure	Level	Pattern	Image	Note
TS17: head	Absent		1E

Structure	Level	Pattern	Image	Note
TS19: cranial ganglion	Present	Uniform	2B
TS19: hindbrain	Present	Uniform	2B

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Weak	Regionally restricted	2C	In masked sections, expression is much less abundant and is only apparent in ventral motor regions of hindbrain.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present	Uniform	2E
TS19: future spinal cord	Present	Uniform	2E
TS19: spinal nerve	Strong	Regionally restricted	2E	Strong expression in both the bundle of His and in the paired spinal nerves along the length of the developing spinal cord.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Weak	Regionally restricted	2F	In masked sections, expression is much less abundant and is only apparent in ventral horns of spinal cord and the oval bundle of His at the dorso-lateral surface of the neural tube.
TS19: spinal nerve	Weak	Regionally restricted	2F	In masked sections, expression is much less abundant and is only apparent in ventral horns of spinal cord and the oval bundle of His at the dorso-lateral surface of the neural tube.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Strong	Regionally restricted	3B	In masked sections, expression is most abundant in ventral regions of the spinal cord.
TS20: cranial ganglion	Absent		3B
TS20: dorsal root ganglion	Absent		3B
TS20: gonad	Absent		3B

Structure	Level	Pattern	Image	Note
TS20: brain	Strong		3D

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted		In masked sections, expression most abundant in lateral cells; less prominent expression in proliferating medial cells.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	3F	In masked sections, expression most abundant in lateral cells; less prominent expression in proliferating medial cells.
TS20: dorsal root ganglion	Absent		3F

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Strong		3G
TS20: dorsal root ganglion	Present		3G
TS20: peripheral nervous system	Present	Regionally restricted	3G	Expression in peripheral nerve fibers.

Structure	Level	Pattern	Image	Note
TS23: cranial ganglion	Present		4D
TS23: dorsal root ganglion	Present		4D
TS23: peripheral nervous system	Present	Regionally restricted	4D	Expression in peripheral nerve fibers throughout the embryo.

Structure	Level	Pattern	Image	Note
TS27: brain	Strong	Uniform	5B

Structure	Level	Pattern	Image	Note
TS27: brain	Present	Non-Uniform	5C
TS27: cerebral cortex	Strong	Regionally restricted	5C	High level of expression in the deeper layers of the cerebral cortex; low level of expression in more superficial layers.
TS27: cerebellar cortex	Weak	Regionally restricted	5C	Low level of expression in more superficial layers of the cerebellar cortex.
TS27: main olfactory bulb	Strong		5C	High level of expression in the olfactory bulb and its associated nerves.