Probe:	Lis1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted	2I	Expression is on the ventricular side of the entire anterior neural tube.
TS19: cerebellum primordium	Present		2I

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Strong	Regionally restricted	2J	Expression is in the ventral hypothalamic primordium and the preoptic area.
TS19: brain	Present	Regionally restricted	2J	Expression is on the ventricular side of the entire anterior neural tube.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2K	Expression is in the subventricular zone.
TS19: telencephalon ventricular layer	Present		2K

Structure	Level	Pattern	Image	Note
TS19: telencephalon ventricular layer	Present		2L

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Present	Scattered	2P , 2O

Structure	Level	Pattern	Image	Note
TS22: cerebellum ventricular layer	Strong		3I
TS22: cerebral cortex subventricular zone	Strong		3I
TS22: cerebral cortex ventricular layer	Strong		3I
TS22: midbrain ventricular layer	Strong		3I
TS22: midbrain lateral wall	Strong	Regionally restricted	3I	Expression is in the subventricular zone.
TS22: inferior colliculus	Strong	Regionally restricted	3I	Expression is in the mantle of the inferior colliculus.
TS22: cerebellum	Strong	Regionally restricted	3I	Expression is at the pial surface.
TS22: thalamus	Present	Regionally restricted	3I	Expression is in the caudal part.
TS22: cerebral cortex marginal layer	Present		3I
TS22: cortical plate	Present		3I
TS22: epithalamus	Present		3I
TS22: pretectum	Absent		3I

Structure	Level	Pattern	Image	Note
TS22: telencephalon ventricular layer	Present	Uniform	3J
TS22: epithalamus	Present		3J
TS22: hypothalamus	Present	Regionally restricted	3J	Expression is in the infundibular and paraventricular region of the hypothalamus.
TS22: telencephalon	Present	Regionally restricted	3J	Expression is in the preoptic area.
TS22: thalamus	Present	Regionally restricted	3J	Expression is in the caudal part.
TS22: pretectum	Absent		3J

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Strong		3K
TS22: cerebral cortex ventricular layer	Strong		3K
TS22: cerebral cortex marginal layer	Present		3K
TS22: cortical plate	Present		3K

Structure	Level	Pattern	Image	Note
TS22: telencephalon ventricular layer	Present	Uniform	3L

Structure	Level	Pattern	Image	Note
TS22: eye	Present		3P , 3O

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex intermediate zone	Strong		4I	Expression is stronger ventrally than dorsally.
TS25: hypothalamus	Present		4I
TS25: telencephalon	Present	Regionally restricted	4I	Expression is in the preoptic area.

Structure	Level	Pattern	Image	Note
TS25: thalamus	Strong		4J
TS25: cerebellum	Present		4J
TS25: hypothalamus	Present		4J
TS25: oculomotor nerve	Present		4J
TS25: telencephalon	Present	Regionally restricted	4J	Expression is in the preoptic area.
TS25: midbrain lateral wall	Present	Regionally restricted	4J	Expression is in the subventricular zone.
TS25: diencephalon	Weak		4J	Expression is in the prethalamus.

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Strong		4K

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex subventricular zone	Present		4L
TS25: cerebral cortex ventricular zone	Present		4L

Structure	Level	Pattern	Image	Note
TS25: retina	Present	Uniform	4O , 4P

Structure	Level	Pattern	Image	Note
TS25: retina	Present		4T , 4S	Co-expression in migrating neurons.

Structure	Level	Pattern	Image	Note
TS28: cortical layer VI	Very strong		5P	Expression is in a set of neurons localized in the deepest part of cortical layer 6.
TS28: main olfactory bulb	Present	Regionally restricted	5P	Expression is in the rostral migratory stream and in the different layers.
TS28: rostral migratory stream	Present	Regionally restricted	5P	Expression is in the rostral migratory stream and in the different layers.
TS28: facial nucleus	Present		5P
TS28: inferior olivary nucleus	Present		5P
TS28: locus coeruleus	Present		5P
TS28: pontine nucleus	Present		5P
TS28: claustrum	Weak		5P
TS28: corpus striatum	Weak		5P
TS28: main olfactory bulb glomerular layer	Weak		5P
TS28: telencephalon septum	Weak		5P

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		5Q
TS28: cerebellum internal granule cell layer	Strong		5Q
TS28: cerebellum external granule cell layer	Present		5Q
TS28: cerebellum molecular layer	Present		5Q

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5R
TS28: cerebellum granule cell layer	Present		5R
TS28: cerebellum molecular layer	Present		5R
TS28: deep cerebellar nucleus	Present		5R

Structure	Level	Pattern	Image	Note
TS28: cortical layer VI	Very strong	Regionally restricted	5S	Expression is in a set of neurons localized in the deepest part of cortical layer 6.
TS28: cortical layer II	Present		5S
TS28: cortical layer III	Present		5S

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Present		5T

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		5U
TS28: cerebellum external granule cell layer	Present		5U
TS28: cerebellum internal granule cell layer	Present		5U
TS28: cerebellum molecular layer	Present		5U

Structure	Level	Pattern	Image	Note
TS28: epithalamus	Present		5V
TS28: habenula	Present		5V
TS28: hypothalamus	Present		5V
TS28: mammillary body	Present		5V
TS28: thalamus	Present		5V
TS28: forebrain	Present	Regionally restricted	5V	Expression is in the preoptic area.
TS28: midbrain	Present	Regionally restricted	5V	Expression is in the precommisural region of the pretectum.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Present		5W
TS28: hippocampus CA3	Present		5W
TS28: dentate gyrus	Weak		5W
TS28: hippocampus CA1	Weak		5W

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		5X