Probe:	Pigpen probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS16: embryo	Present	Widespread

Structure	Level	Pattern	Image	Note
TS19: jaw primordium mesenchyme	Present	Graded	2A	Expression was detected in the dental mesenchyme in a buccal to lingual gradient.

Structure	Level	Pattern	Image	Note
TS21: tooth epithelium	Strong		2B
TS21: tooth mesenchyme	Present		2B

Structure	Level	Pattern	Image	Note
TS22: cervical loop	Strong	Regionally restricted	2C	Expression was intense in the proliferating epithelia of the cervical loops.
TS22: enamel knot	Ambiguous		2C	Expression was down-regulated in the enamel knot cells.
TS22: tooth mesenchyme	Present		2C

Structure	Level	Pattern	Image	Note
TS23: cervical loop	Present		2D
TS23: dental papilla	Present		2D

Structure	Level	Pattern	Image	Note
TS17: optic cup	Present	Regionally restricted	3A	Expression was detected in the neural retina and the retinal pigmented epithelium.
TS17: optic stalk	Present		3A

Structure	Level	Pattern	Image	Note
TS21: extrinsic ocular pre-muscle mass	Present		3B
TS21: eyelid mesenchyme	Present		3B
TS21: neural retinal epithelium	Present		3B
TS21: pigmented retinal epithelium	Present		3B
TS21: lens epithelium	Present	Regionally restricted	3B	Expression was restricted to the anterior proliferating lens epithelium, and no expression was detected in the more posterior differentiated lens fibers.

Structure	Level	Pattern	Image	Note
TS22: extrinsic ocular muscle	Present		3C
TS22: eyelid mesenchyme	Present		3C
TS22: neural retinal epithelium	Present		3C
TS22: pigmented retinal epithelium	Present		3C
TS22: lens epithelium	Present	Regionally restricted	3C	Expression was restricted to the anterior proliferating lens epithelium, and no expression was detected in the more posterior differentiated lens fibers.

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present	Regionally restricted	3D	Expression was stronger in the proliferating ventricular layer of the neural retina, and expression was lost from the differentiated ganglion layer.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	4A	Expression was detected in the neural epithelium of brain ventricles.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		4B

Structure	Level	Pattern	Image	Note
TS21: incisor	Present		4C
TS21: olfactory epithelium	Present		4C
TS21: tongue	Present		4C
TS21: vibrissa	Present		4C

Structure	Level	Pattern	Image	Note
TS21: tooth	Present		4D

Structure	Level	Pattern	Image	Note
TS21: vibrissa	Present		4E

Structure	Level	Pattern	Image	Note
TS22: submandibular gland primordium	Present		4F

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present	Regionally restricted	4G	Expression was enriched in the ureteric buds.
TS21: mesenchyme of metanephros	Present		4G	Expression was in the condensing metanephric mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: lobar bronchus epithelium	Present	Regionally restricted	4H	Expression was restricted to the epithelium of the forming bronchioles.
TS21: lung mesenchyme	Present		4H