Antibody:	STC1 antibody
Detection system:	Secondary antibody coupled to Texas Red
Assay notes:	Fixative was 4% formaldehyde.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present		1A

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Strong		1B	Expression is in the peri-notochordal mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: basisphenoid cartilage condensation	Present		1C

Structure	Level	Pattern	Image	Note
TS17: sclerotome	Absent		1D

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Present		1E

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Present		1F

Structure	Level	Pattern	Image	Note
TS23: intervertebral disc	Strong		1G
TS23: vertebra	Present		1G

Structure	Level	Pattern	Image	Note
TS22: tail sclerotome	Present		2A	Expression is prechondrocytes in the condensing sclerotome.

Structure	Level	Pattern	Image	Note
TS22: vertebral cartilage condensation	Present	Regionally restricted	2B	Expression is absent in young chondrocytes (caudal veretebrae) and is present in prehypertrophic chondrocytes (rostral vertebrea)

Structure	Level	Pattern	Image	Note
TS22: vertebral cartilage condensation	Present	Regionally restricted	2C	Expression is absent in young chondrocytes (caudal veretebrae) and is present in prehypertrophic chondrocytes (rostral vertebrea).

Structure	Level	Pattern	Image	Note
TS22: thoracic vertebral cartilage condensation	Weak		2D
TS22: esophagus epithelium	Present		2D

Structure	Level	Pattern	Image	Note
TS23: thoracic vertebra	Present	Regionally restricted	2E	Expression is in the layer of chondrocytes surrounding the ossifying core of the vertebral body.

Structure	Level	Pattern	Image	Note
TS23: vertebra	Present	Regionally restricted	3A left	Expression is in a circular layer of chondrocytes between the proliferative and hypertrophic zones outside the central core of hypertrophic cartilage.

Structure	Level	Pattern	Image	Note
TS23: ulna	Present	Regionally restricted	3B left	Expression is in the transitional layer between the proliferating and hypertrophic zones, and is absent in either the proliferating or hypertrophic zones, or in the zone of ossification.

Structure	Level	Pattern	Image	Note
TS23: otic capsule	Present	Regionally restricted	3C	Expression is in chondrocytes in most of the capsule.

Structure	Level	Pattern	Image	Note
TS23: nasal septum	Present	Regionally restricted	3D	Expression is in chondrocytes.

Structure	Level	Pattern	Image	Note
TS21: Meckel's cartilage	Present		3E
TS21: oral epithelium	Present		3E
TS21: mandible mesenchyme	Present	Regionally restricted	3E	Expression is in the mesenchymal condensation.

Structure	Level	Pattern	Image	Note
TS21: maxilla	Present

Structure	Level	Pattern	Image	Note
TS20: myotome	Present	Regionally restricted	4A	Expression is in myoblasts of the condensed myotome.

Structure	Level	Pattern	Image	Note
TS21: myotome	Strong	Regionally restricted	4B	Expression is in myoblasts of the condensed myotome.

Structure	Level	Pattern	Image	Note
TS23: skeletal muscle	Present	Patchy	4C	Expression is in myotubes.

Structure	Level	Pattern	Image	Note
TS21: skeletal muscle	Present	Regionally restricted	4D	Expression is in muscles in the back.

Structure	Level	Pattern	Image	Note
TS21: anterior abdominal wall muscle	Present		4E

Structure	Level	Pattern	Image	Note
TS21: limb musculature	Present		4F

Structure	Level	Pattern	Image	Note
TS13: heart	Present		5A

Structure	Level	Pattern	Image	Note
TS19: atrium cardiac muscle	Present		5B1/B2
TS19: ventricle cardiac muscle	Present		5B1/B2
TS19: endocardial cushion	Absent		5B1/B2

Structure	Level	Pattern	Image	Note
TS21: ventricle cardiac muscle	Present	Regionally restricted	5C	Expression is in the ventricular trabeculae.

Structure	Level	Pattern	Image	Note
TS23: esophagus	Present	Regionally restricted	5D	Expression is in the basal layer of the stratified mucous epithelium and is absent in smooth muscle.

Structure	Level	Pattern	Image	Note
TS23: intestine epithelium	Absent		5E
TS23: intestine muscularis	Absent		5E

Structure	Level	Pattern	Image	Note
TS23: heart	Trace