Antibody:	Anti-PGP9.5
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS28: circumvallate papillae	Present	Regionally restricted	2A	Expression in thin nerve fibers in the lamina propria forming nerve plexus beneath the taste buds, with some penetrating the taste buds of the trench wall epithelium. Expression in round- to oval-shaped ganglionic cells in the lamina propria.
TS28: taste bud	Present	Regionally restricted	2A	Expression also observed in spindle-shaped taste cells.

Structure	Level	Pattern	Image	Note
TS28: circumvallate papillae	Present	Regionally restricted	2B	Expression at the border of apical epithelium and trench wall epithelium. and in nerve fibers penetrating the apical epithelium, but not in cells.

Structure	Level	Pattern	Image	Note
TS28: taste bud	Present	Regionally restricted	2C	Expression in spindle-shaped taste cells.

Structure	Level	Pattern	Image	Note
TS28: circumvallate papillae	Present	Regionally restricted		Authors report expression at the border of the outer trench wall epithelium and lingual epithelium.

Structure	Level	Pattern	Image	Note
TS20: tongue epithelium	Present	Regionally restricted	3A	Strong expression in lamina propria beneath the newly formed papillary core. Expression in thick nerve bundles, presumably the glossopharyngeal nerve. No expression in nerve fibers penetrating the apical epithelium.

Structure	Level	Pattern	Image	Note
TS21: tongue epithelium	Present	Regionally restricted

Structure	Level	Pattern	Image	Note
TS22: circumvallate papillae	Present	Regionally restricted

Structure	Level	Pattern	Image	Note
TS23: circumvallate papillae	Present	Regionally restricted	4	Expression in nerve fibers penetrating apical epithelium, straight from nerve plexus in lamina propria, but not penetrating lateral trench epithelium. At apical epithelium, expression in an occasional round- to oval-shaped cell

Structure	Level	Pattern	Image	Note
TS25: circumvallate papillae	Present	Regionally restricted	5A	Expression in thin nerve fibers penetrating the inner lateral epithelium of the trench, and at the border of the outer lateral epithelium and lingual epithelium.

Structure	Level	Pattern	Image	Note
TS26: circumvallate papillae	Present	Regionally restricted

Structure	Level	Pattern	Image	Note
TS27: circumvallate papillae	Present	Regionally restricted	6A	Expression in nerve fibers penetrating outer lateral trench wall epithelium. Expression in a round- to oval-shaped cell in the apical epithelium.

Structure	Level	Pattern	Image	Note
TS28: circumvallate papillae	Present	Regionally restricted	8	Expression in round- or oval-shaped cell in inner trench wall epithelium, latera changing to a spindle-like appearance. Expression in apical epithelium disappeared.

Structure	Level	Pattern	Image	Note
TS28: taste bud	Present	Regionally restricted	9A	Expression in many cells in the taste bud at the trench wall epithelium. Expression in increasing number of taste cells.
TS28: circumvallate papillae	Present	Regionally restricted	9A	Expression in nerve fibers in the lamina propria, with number of expressing cells decreasing slightly but still higher than that seen in adults (compare with Figure 2A).

Structure	Level	Pattern	Image	Note
TS28: circumvallate papillae	Present	Regionally restricted		Authors state the distribution of expression was almost identical to that seen in adults.