Probe:	Vezf1 probe C
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS10: yolk sac mesoderm	Strong	Uniform	5A/B	Onset of expression detected at the primitive streak stage. Strong, uniform expression localized to the extraembryonic mesodermal component of the visceral yolk sac.
TS10: amniotic fold	Absent		5A/B	No expression in the ectoplacental cone, amnion, chorion, or regions of the embryo proper.
TS10: ectoplacental cone	Absent		5A/B	No expression in the ectoplacental cone, amnion, chorion, or regions of the embryo proper.
TS10: embryo	Absent		5A/B	No expression in the ectoplacental cone, amnion, chorion, or regions of the embryo proper.

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch mesenchyme	Strong	Regionally restricted	5C/D	Patches of intense expression in regions of angioblast differentiation into the primary vascular plexus. Strong expression in capillary structures of the cephalic mesenchyme and first branchial arch, the inner lining of the dorsal aorta and its communication with the first branchial arch artery, the endocardium, and the yolk sac blood islands.
TS13: blood island	Strong	Regionally restricted	5C/D	Patches of intense expression in regions of angioblast differentiation into the primary vascular plexus. Strong expression in capillary structures of the cephalic mesenchyme and first branchial arch, the inner lining of the dorsal aorta and its communication with the first branchial arch artery, the endocardium, and the yolk sac blood islands.
TS13: dorsal aorta	Strong	Regionally restricted	5C/D	Patches of intense expression in regions of angioblast differentiation into the primary vascular plexus. Strong expression in capillary structures of the cephalic mesenchyme and first branchial arch, the inner lining of the dorsal aorta and its communication with the first branchial arch artery, the endocardium, and the yolk sac blood islands.
TS13: endocardial tube	Strong	Regionally restricted	5C/D	Patches of intense expression in regions of angioblast differentiation into the primary vascular plexus. Strong expression in capillary structures of the cephalic mesenchyme and first branchial arch, the inner lining of the dorsal aorta and its communication with the first branchial arch artery, the endocardium, and the yolk sac blood islands.
TS13: head mesenchyme	Strong	Regionally restricted	5C/D	Patches of intense expression in regions of angioblast differentiation into the primary vascular plexus. Strong expression in capillary structures of the cephalic mesenchyme and first branchial arch, the inner lining of the dorsal aorta and its communication with the first branchial arch artery, the endocardium, and the yolk sac blood islands.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Strong	Regionally restricted	5E	High levels of expression detected in the putative angioblasts of the head mesenchyme.
TS17: vascular element	Strong	Regionally restricted	5E	High levels of expression detected in the putative angioblasts of the meningeal plexus surrounding the brain and spinal cord, dorsal aorta, heart chambers, truncus arteriosus, and umbilical vein.

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Strong	Regionally restricted	5G/H	Strong expression in capillaries sprouting from the dorsal aorta. Expression restricted to a single layer of presumptive endothelium in the larger vessels.
TS17: blood	Absent		5G/H	No expression in circulating nucleated blood cells trapped in sections of the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS17: endocardial tube	Strong	Regionally restricted	5I/J	Expression restricted to a single layer of presumptive endothelium.

Structure	Level	Pattern	Image	Note
TS17: intersomitic artery	Strong	Regionally restricted	5K/L	Expression restricted to a single layer of presumptive endothelium.

Structure	Level	Pattern	Image	Note
TS17: branchial arch artery	Strong		5M/N

Structure	Level	Pattern	Image	Note
TS17: lung rudiment vascular element	Present	Patchy	5O	Expression in sprouting capillaries in the lung rudiment. Small patches of expression in what appear to be interstitial capillaries between mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS17: urinary system	Present	Regionally restricted	5P	In the kidney rudiment, small patches of expression in what appear to be interstitial capillaries between mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS21: vascular system	Present	Widespread	6A	Sustained expression throughout the vascular plexus.
TS21: central nervous system	Present	Punctate	6A	Punctate expression throughout the central nervous system and in the craniofacial mesenchyme.
TS21: head mesenchyme	Present	Punctate	6A	Punctate expression throughout the central nervous system and in the craniofacial mesenchyme.
TS21: 4th ventricle choroid plexus	Present	Regionally restricted	6A
TS21: heart ventricle	Strong		6A
TS21: liver vasculature	Strong	Regionally restricted	6A	High expression in capillaries of the liver and lung.
TS21: lung vascular element	Strong	Regionally restricted	6A	High expression in capillaries of the liver and lung.

Structure	Level	Pattern	Image	Note
TS21: developing vasculature of metanephros	Strong	Regionally restricted	6B	High expression in capillaries of the kidney
TS21: vasculature	Strong	Regionally restricted	6B	Strong expression in intervertebral vessels.

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Present	Clusters	6C	Clusters of expressing cells in mesenchyme of the choroid plexus.

Structure	Level	Pattern	Image	Note
TS21: liver vasculature	Strong	Regionally restricted	6D	High expression in capillaries of the liver. Expression in fetal liver appeared to be restricted to interstitial capillaries. No expression in primitive hematopoietic cells of the liver.

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present	Punctate	6E	Punctate expression throughout the central nervous system and in the craniofacial mesenchyme.
TS21: head mesenchyme	Present	Punctate	6E	Punctate expression throughout the central nervous system and in the craniofacial mesenchyme.
TS21: 4th ventricle choroid plexus	Present	Regionally restricted	6E
TS21: dorsal aorta	Strong	Regionally restricted	6E	Strong expression in the dorsal aorta and surrounding capillaries.
TS21: heart ventricle	Strong		6E
TS21: liver vasculature	Strong	Regionally restricted	6E	High expression in capillaries of the liver and lung.
TS21: lung vascular element	Strong	Regionally restricted	6E	High expression in capillaries of the liver and lung.

Structure	Level	Pattern	Image	Note
TS21: dorsal aorta	Strong	Regionally restricted	6F	Strong expression in the dorsal aorta and surrounding capillaries.
TS21: blood	Absent		6F	No expression in circulating nucleated blood cells trapped in section of the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS21: lung vascular element	Strong		6G	High expression in capillaries of the lung.

Structure	Level	Pattern	Image	Note
TS21: heart ventricle	Strong		6H
TS21: blood	Absent		6H	No expression in circulating nucleated blood cells trapped in section of the heart.