Hoxb4 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:226028 Lewandowski JP, et al., Spatiotemporal regulation of GLI target genes in the mammalian limb bud. Dev Biol. 2015 Oct 1;406(1):92-103
Assay type:	RNA in situ
MGI Accession ID:	MGI:5823635
Gene symbol:	Hoxb4
Gene name:	homeobox B4

Probe:	Hoxb4 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen Hoxb4_b86-flp_E10.0a_RF_JL: embryonic day 10.0 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Hoxb4_b86-flp_E10.0a_RF_JL	Staining is observed in the proximal-anterior limb bud.

Specimen Hoxb4_b86-flp_E11.5a_RF_JL: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Hoxb4_b86-flp_E11.5a_RF_JL	Domains of staining are similar to those seen at E10.5.

Specimen Hoxb4_b86-flp_E11.5b_RF_JL: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Hoxb4_b86-flp_E11.5b_RF_JL	Domains of staining are similar to those seen at E10.5.

Specimen Hoxb4_b86-flp_E11.5a_HLR_JL: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		Hoxb4_b86-flp_E11.5a_HLR_JL

Specimen Hoxb4_b86-flp_E10.0a_JL: embryonic day 10.0 (more

)

Structure	Level	Pattern	Image	Note
TS17: somite	Strong		Hoxb4_b86-flp_E10.0a_JL
TS17: central nervous system	Strong		Hoxb4_b86-flp_E10.0a_JL

Specimen Hoxb4_b86-flp_E11.5a_JL: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Hoxb4_b86-flp_E11.5a_JL	Staining is similar to that observed at E10.5. At that time it was seen in the somites and central nervous system.

Specimen Hoxb4_b86-flp_E11.5b_JL: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Hoxb4_b86-flp_E11.5b_JL	Staining is similar to that observed at E10.5. At that time it was seen in the somites and central nervous system.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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