Antibody:	STC antibody
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: mesonephric tubule	Present		1B	Expression in both the mesenchyme and particularly in tubular cells.
TS17: mesonephros mesenchyme	Present		1B	Expression in both the mesenchyme and particularly in tubular cells.
TS17: primordial germ cell of gonad	Present		1B	Expression in putative primordial germ cells within the gonadal component of the urogenital ridge.

Structure	Level	Pattern	Image	Note
TS20: nephrogenic interstitium	Present		1D	Expression in metanephrogenic blastema cells.
TS20: primitive collecting duct	Present	Regionally restricted	1D	Expression in epithelial cells of the ureteric bud and other developing tubules.

Structure	Level	Pattern	Image	Note
TS22: collecting duct	Present	Regionally restricted	1E	Expression most evident in ureteric bud epithelial cells.

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Present	Regionally restricted	1G	Expression most highly concentrated in ureteric bud epithelial cells.
TS23: metanephros	Present	Regionally restricted	1G	Expression in cells of other nephron segments.

Structure	Level	Pattern	Image	Note
TS24: cortical early proximal tubule	Present		1H
TS24: maturing glomerular tuft	Present		1H

Structure	Level	Pattern	Image	Note
TS25: inner medullary collecting duct	Strong		2B	High levels of expression in collecting ducts of outer and inner medullary kidney.
TS25: outer medullary collecting duct	Strong		2B	High levels of expression in collecting ducts of outer and inner medullary kidney.
TS25: ureter	Present	Regionally restricted	2B	In the ureter, expression in condensing mesenchymal cells surrounding the ureter epithelium.
TS25: urothelium of ureter	Strong	Regionally restricted	2B	Much higher expression in adjacent luminal epithelial cells.

Structure	Level	Pattern	Image	Note
TS25: urothelium of ureter	Present	Regionally restricted	2B inset	Expression in luminal epithelial cells.

Structure	Level	Pattern	Image	Note
TS26: collecting duct	Present	Widespread	2D	Expression throughout all branches of the collecting duct system.
TS26: glomerular tuft	Strong		2D	High levels of expression in glomeruli.
TS26: nephron	Strong	Regionally restricted	2D	High levels of expression in proximal nephron segments.

Structure	Level	Pattern	Image	Note
TS22: primary sex cord	Strong	Regionally restricted	2H	Expression most abundant in the sex cord cells.

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Strong	Regionally restricted	2H inset	Expression in sex cords confined to the nuclei of developing gonocytes.

Structure	Level	Pattern	Image	Note
TS25: testis	Present		3B	Authors report a change in protein expression in the testis.
TS25: ductus deferens	Present	Regionally restricted	3B

Structure	Level	Pattern	Image	Note
TS25: seminiferous cord	Present	Regionally restricted	3C
TS25: germ cell of testis	Present	Regionally restricted	3C	Expression most evident in a subpopulation of developing gonocytes, with others devoid of expression.

Structure	Level	Pattern	Image	Note
TS25: interstitium of the testis	Present	Regionally restricted	3D	Authors describe a clearcut hypertrophy in a select group of interstitial cells giving them the appearance of being more intensely stained.

Structure	Level	Pattern	Image	Note
TS20: nephric duct, mesonephric portion	Present	Regionally restricted	3F	Expression confined for the most part to tubular epithelial cells.

Structure	Level	Pattern	Image	Note
TS25: ovary	Weak	Diffuse	3H
TS25: paramesonephric duct	Present	Regionally restricted	3H	Expression in the developing Mullerian ducts confined to ductal epithelial cells.

Structure	Level	Pattern	Image	Note
TS19: mesenchyme of metanephros	Present	Regionally restricted		Expression in mesenchyme cells on the rostral and caudal tips of the emerging metanephric kidney.
TS19: mesonephric tubule	Present	Regionally restricted		Expression also in tubular epithelial cells of the degenerating mesonephros.
TS19: nephric duct, mesonephric portion	Present
TS19: urogenital ridge	Present	Regionally restricted		Expression in the rostral aspect of the mesonephros within the area corresponding to the genital ridge.

Structure	Level	Pattern	Image	Note
TS21: nephrogenic interstitium	Present			Authors state that the pattern of expression seen at E12.5 persisted to E13.5.
TS21: primitive collecting duct	Present	Regionally restricted		Authors state that the pattern of expression seen at E12.5 persisted to E13.5.

Structure	Level	Pattern	Image	Note
TS26: ureter	Strong	Regionally restricted		Intense expression in ureter epithelium.