Gpam Gene Detail

Summary

• Symbol
  Gpam
• Name
  glycerol-3-phosphate acyltransferase, mitochondrial
• Synonyms
  GPAT1

• Feature Type
  protein coding gene
• IDs
  MGI:109162
  NCBI Gene: 14732
• Alliance
  gene page
• Transcription Start Sites
  16 TSS
• Candidate for QTL
  6 QTL

Location &
Maps

• Sequence Map
  Chr19:55056067-55115666 bp, - strand

  From NCBI annotation of GRCm39
  

• View this region in JBrowse
  

• Genome Browsers
  Ensembl | UCSC | NCBI

• Genetic Map
  Chromosome 19, 50.81 cM
• Mapping Data
  6 experiments

Strain
Comparison

• SNPs within 2kb
  1891 from dbSNP Build 142
• Strain Annotations
  18
• RFLP
  1

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_109162	protein coding gene	Chr19:55055700-55115670 (-)
129S1/SvImJ	MGP_129S1SvImJ_G0025254	protein coding gene	Chr19:55327591-55364739 (-)
A/J	MGP_AJ_G0025230	protein coding gene	Chr19:52603779-52639068 (-)
AKR/J	MGP_AKRJ_G0025199	protein coding gene	Chr19:54262920-54292204 (-)
BALB/cJ	MGP_BALBcJ_G0025225	protein coding gene	Chr19:52669213-52698544 (-)
C3H/HeJ	MGP_C3HHeJ_G0024985	protein coding gene	Chr19:54486984-54523249 (-)
C57BL/6NJ	MGP_C57BL6NJ_G0025668	protein coding gene	Chr19:56678023-56715762 (-)
CAROLI/EiJ	MGP_CAROLIEiJ_G0023080	protein coding gene	Chr19:51539072-51569734 (-)
CAST/EiJ	MGP_CASTEiJ_G0024450	protein coding gene	Chr19:54481088-54513424 (-)
CBA/J	MGP_CBAJ_G0024965	protein coding gene	Chr19:59485969-59523593 (-)
DBA/2J	MGP_DBA2J_G0025097	protein coding gene	Chr19:52403808-52433708 (-)
FVB/NJ	MGP_FVBNJ_G0025058	protein coding gene	Chr19:52101749-52139606 (-)
LP/J	MGP_LPJ_G0025183	protein coding gene	Chr19:55204800-55234443 (-)
NOD/ShiLtJ	MGP_NODShiLtJ_G0025091	protein coding gene	Chr19:58860516-58894817 (-)
NZO/HlLtJ	MGP_NZOHlLtJ_G0025726	protein coding gene	Chr19:54469743-54504677 (-)
PWK/PhJ	MGP_PWKPhJ_G0024198	protein coding gene	Chr19:52324650-52362855 (-)
SPRET/EiJ	MGP_SPRETEiJ_G0024001	protein coding gene	Chr19:53149373-53181477 (-)
WSB/EiJ	MGP_WSBEiJ_G0024520	protein coding gene	Chr19:54597649-54628813 (-)

Homology

• Human Ortholog
  GPAM, glycerol-3-phosphate acyltransferase, mitochondrial

• Vertebrate Orthologs
  3

Vertebrate Orthology Source

Alliance of Genome Resources

• Human Ortholog
  GPAM, glycerol-3-phosphate acyltransferase, mitochondrial
  
• Synonyms
  GPAT, GPAT1
• Links
  HGNC:24865

  NCBI Gene ID: 57678

  neXtProt AC: NX_Q9HCL2

  UniProt: Q9HCL2

  
  
• Chr Location
  10q25.2; chr10:112149865-112227677 (-)  GRCh38

---

• MGI Vertebrate Homology
  Gpam stringent orthology
  1 human;1 mouse;1 rat;1 zebrafish
  
• HCOP
  vertebrate homology predictions: GPAM
• Protein SuperFamily
  GPAT_DHAPAT
• Gene Tree
  Gpam
  

Mutations,
Alleles, and
Phenotypes

• Phenotype Summary
  17 phenotypes from 3 alleles in 3 genetic backgrounds
  23 phenotype references
  

• All Mutations and Alleles
  14
• Endonuclease-mediated
  5
• Targeted
  9
• Incidental Mutations
  Mutagenetix , APF , CvDC
• Find Mice (IMSR)
  53 strains or lines available
• Comparison Matrix
  Gene Expression + Phenotype

Homozygous mutant mice weighed less than controls and showed reduced triacylglycerol levels in the liver and plasma. The glycerolipid fatty acid composition is also disrupted in mutant mice.

Gene Ontology
(GO)
Classifications

• All GO Annotations
  46
• GO References
  21

Expression

• Assay Results
  907
• Tissues
  151
• cDNA Data
  58
• Literature Summary
  10

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

• Other Mouse Links
  Allen Institute
  GEO
  Expression Atlas
• Other Vertebrate Links
  GEISHA GPAM
  Xenbase gpam

Sequences &
Gene Models

• All Sequences
  58
• RefSeq
  15
• UniProt
  4
• CCDS
  CCDS29906.1

• Ensembl
  ENSMUSG00000024978 (Evidence)
• NCBI Gene
  14732

Representative Sequences			Length	Strain/Species
genomic	14732	NCBI Gene Model | MGI Sequence Detail	59600	C57BL/6J
transcript	NM_001356285	RefSeq | MGI Sequence Detail	6242	ZRU/MplStud
polypeptide	Q61586	UniProt | EBI | MGI Sequence Detail	827	Not Applicable

Protein
Information

• UniProt
  4 Sequences
• Protein Ontology
  PR:000008159 glycerol-3-phosphate acyltransferase 1, mitochondrial
  (term hierarchy)
• EC
  2.3.1.15
• InterPro Domains
  IPR028354 Glycerol-3-phosphate acyltransferase, PlsB
  IPR022284 Glycerol-3-phosphate O-acyltransferase/Dihydroxyacetone phosphate acyltransferase
  IPR041728 GPAT/DHAPAT, acyltransferase domain
  IPR045520 GPAT/DHAPAT, C-terminal domain
  IPR002123 Phospholipid/glycerol acyltransferase
• GlyGen
  Q61586 1 site, 1 O-linked glycan (1 site)

Molecular
Reagents

• All nucleic 62

  Genomic 1

  cDNA 58

  Primer pair 2

  Other 1

  
  

  Microarray probesets 4

Other
Accession IDs

MGD-MRK-38206

References

• Summaries
  All 73

  Developmental Gene Expression 10

  Gene Ontology 21

  Phenotypes 23
• Earliest
  J:24536 Shin DH, et al., Transcriptional regulation of p90 with sequence homology to Escherichia coli glycerol-3-phosphate acyltransferase. J Biol Chem. 1991 Dec 15;266(35):23834-9
• Latest
  J:344186 Munoz-Gallardo MDM, et al., miR-195b is required for proper cellular homeostasis in the elderly. Sci Rep. 2024 Jan 8;14(1):810

TSS for Gpam:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr159855	Chr19:55115679-55115689 (-)	-18 bp
Tssr159854	Chr19:55115612-55115675 (-)	22 bp
Tssr159853	Chr19:55115598-55115611 (-)	61 bp
Tssr159852	Chr19:55108242-55108247 (-)	7,421 bp
Tssr159851	Chr19:55105433-55105446 (-)	10,226 bp
Tssr159850	Chr19:55103063-55103083 (-)	12,593 bp
Tssr159849	Chr19:55091773-55091781 (-)	23,889 bp
Tssr159848	Chr19:55091557-55091578 (-)	24,098 bp
Tssr159847	Chr19:55091524-55091531 (-)	24,138 bp
Tssr159846	Chr19:55091502-55091521 (-)	24,154 bp
Tssr159845	Chr19:55091462-55091496 (-)	24,187 bp
Tssr159844	Chr19:55087849-55087924 (-)	27,779 bp
Tssr159843	Chr19:55085316-55085320 (-)	30,348 bp
Tssr159842	Chr19:55085173-55085186 (-)	30,486 bp
Tssr159841	Chr19:55084156-55084196 (-)	31,490 bp
Tssr159840	Chr19:55064643-55064647 (-)	51,021 bp

Gpam is Candidate Gene for:

QTL	Genetic Location*	Genome Location (GRCm39)	Reference	QTL Note
Afw8	Chr19, syntenic		J:99477	Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes. Potential candidate genes for Iba4 (20 cM) on mouse Chromosome 19 as identified by ExQuest are Aldh1a1 (12 cM), Aldh1a7, Vldlr (20 cM), and Plce1. Vldlr exhibits 15.6-fold increased expression in the liver of Tally Ho compared to C57BL/6 animals. For QTL Afw8 (26 cM), potential candidate genes Pi4k2a, Cpn1, and Elovl3 (47 cM) were identified. For QTLs Nobq2 (47 cM) and Bglq13 (51 cM), potential candidate genes Ins1 (49 cM), Gpam (52 cM), and Acsl5 were identified. For QTLs Tanidd1 (50 cM) and T2dm2 (53 cM), potential candidate genes Gfra1, Pnlip (29 cM), Pnliprp1 (29 cM), and Pnliprp2 were identified. Pnliprp2 exhibits 21.8-fold decreased expression in the pancreas of Tally Ho animals in response to a 4% fat diet.
Bglq13	Chr19, syntenic		J:99477	Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes. Potential candidate genes for Iba4 (20 cM) on mouse Chromosome 19 as identified by ExQuest are Aldh1a1 (12 cM), Aldh1a7, Vldlr (20 cM), and Plce1. Vldlr exhibits 15.6-fold increased expression in the liver of Tally Ho compared to C57BL/6 animals. For QTL Afw8 (26 cM), potential candidate genes Pi4k2a, Cpn1, and Elovl3 (47 cM) were identified. For QTLs Nobq2 (47 cM) and Bglq13 (51 cM), potential candidate genes Ins1 (49 cM), Gpam (52 cM), and Acsl5 were identified. For QTLs Tanidd1 (50 cM) and T2dm2 (53 cM), potential candidate genes Gfra1, Pnlip (29 cM), Pnliprp1 (29 cM), and Pnliprp2 were identified. Pnliprp2 exhibits 21.8-fold decreased expression in the pancreas of Tally Ho animals in response to a 4% fat diet.
Iba4	Chr19, 20.99 cM	Chr19:26305715-26305821	J:99477	Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes. Potential candidate genes for Iba4 (20 cM) on mouse Chromosome 19 as identified by ExQuest are Aldh1a1 (12 cM), Aldh1a7, Vldlr (20 cM), and Plce1. Vldlr exhibits 15.6-fold increased expression in the liver of Tally Ho compared to C57BL/6 animals. For QTL Afw8 (26 cM), potential candidate genes Pi4k2a, Cpn1, and Elovl3 (47 cM) were identified. For QTLs Nobq2 (47 cM) and Bglq13 (51 cM), potential candidate genes Ins1 (49 cM), Gpam (52 cM), and Acsl5 were identified. For QTLs Tanidd1 (50 cM) and T2dm2 (53 cM), potential candidate genes Gfra1, Pnlip (29 cM), Pnliprp1 (29 cM), and Pnliprp2 were identified. Pnliprp2 exhibits 21.8-fold decreased expression in the pancreas of Tally Ho animals in response to a 4% fat diet.
Nobq2	Chr19, 40.53 cM	Chr19:47897880-47897988	J:99477	Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes. Potential candidate genes for Iba4 (20 cM) on mouse Chromosome 19 as identified by ExQuest are Aldh1a1 (12 cM), Aldh1a7, Vldlr (20 cM), and Plce1. Vldlr exhibits 15.6-fold increased expression in the liver of Tally Ho compared to C57BL/6 animals. For QTL Afw8 (26 cM), potential candidate genes Pi4k2a, Cpn1, and Elovl3 (47 cM) were identified. For QTLs Nobq2 (47 cM) and Bglq13 (51 cM), potential candidate genes Ins1 (49 cM), Gpam (52 cM), and Acsl5 were identified. For QTLs Tanidd1 (50 cM) and T2dm2 (53 cM), potential candidate genes Gfra1, Pnlip (29 cM), Pnliprp1 (29 cM), and Pnliprp2 were identified. Pnliprp2 exhibits 21.8-fold decreased expression in the pancreas of Tally Ho animals in response to a 4% fat diet.
T2dm2	Chr19, 50.72 cM	Chr19:55376050-55376167	J:99477	Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes. Potential candidate genes for Iba4 (20 cM) on mouse Chromosome 19 as identified by ExQuest are Aldh1a1 (12 cM), Aldh1a7, Vldlr (20 cM), and Plce1. Vldlr exhibits 15.6-fold increased expression in the liver of Tally Ho compared to C57BL/6 animals. For QTL Afw8 (26 cM), potential candidate genes Pi4k2a, Cpn1, and Elovl3 (47 cM) were identified. For QTLs Nobq2 (47 cM) and Bglq13 (51 cM), potential candidate genes Ins1 (49 cM), Gpam (52 cM), and Acsl5 were identified. For QTLs Tanidd1 (50 cM) and T2dm2 (53 cM), potential candidate genes Gfra1, Pnlip (29 cM), Pnliprp1 (29 cM), and Pnliprp2 were identified. Pnliprp2 exhibits 21.8-fold decreased expression in the pancreas of Tally Ho animals in response to a 4% fat diet.
Tanidd1	Chr19, 48.46 cM	Chr19:53752597-53752718	J:99477	Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes. Potential candidate genes for Iba4 (20 cM) on mouse Chromosome 19 as identified by ExQuest are Aldh1a1 (12 cM), Aldh1a7, Vldlr (20 cM), and Plce1. Vldlr exhibits 15.6-fold increased expression in the liver of Tally Ho compared to C57BL/6 animals. For QTL Afw8 (26 cM), potential candidate genes Pi4k2a, Cpn1, and Elovl3 (47 cM) were identified. For QTLs Nobq2 (47 cM) and Bglq13 (51 cM), potential candidate genes Ins1 (49 cM), Gpam (52 cM), and Acsl5 were identified. For QTLs Tanidd1 (50 cM) and T2dm2 (53 cM), potential candidate genes Gfra1, Pnlip (29 cM), Pnliprp1 (29 cM), and Pnliprp2 were identified. Pnliprp2 exhibits 21.8-fold decreased expression in the pancreas of Tally Ho animals in response to a 4% fat diet.

*cM position of peak correlated region/marker