Cflar Gene Detail

Summary

• Symbol
  Cflar
• Name
  CASP8 and FADD-like apoptosis regulator
• Synonyms
  2310024N18Rik, A430105C05Rik, Cash, Casper, c-Flip, Flip

• Feature Type
  protein coding gene
• IDs
  MGI:1336166
  NCBI Gene: 12633
• Alliance
  gene page
• Transcription Start Sites
  48 TSS

Location &
Maps

• Sequence Map
  Chr1:58750667-58798043 bp, + strand

  From Ensembl annotation of GRCm39
  

• View this region in JBrowse
  

• Genome Browsers
  Ensembl | UCSC | NCBI

• Genetic Map
  Chromosome 1, 29.16 cM
• Mapping Data
  7 experiments

Strain
Comparison

• SNPs within 2kb
  1184 from dbSNP Build 142
• Strain Annotations
  18

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_1336166	protein coding gene	Chr1:58750353-58798043 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0016057	protein coding gene	Chr1:58634301-58686115 (+)
A/J	MGP_AJ_G0016040	protein coding gene	Chr1:56569516-56619785 (+)
AKR/J	MGP_AKRJ_G0015996	protein coding gene	Chr1:58113824-58159463 (+)
BALB/cJ	MGP_BALBcJ_G0015995	protein coding gene	Chr1:56605943-56651812 (+)
C3H/HeJ	MGP_C3HHeJ_G0015827	protein coding gene	Chr1:58386931-58436244 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0016448	protein coding gene	Chr1:60972615-61017995 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0014169	protein coding gene	Chr1:52463656-52503455 (+)
CAST/EiJ	MGP_CASTEiJ_G0015409	protein coding gene	Chr1:57570090-57622518 (+)
CBA/J	MGP_CBAJ_G0015799	protein coding gene	Chr1:62808460-62862689 (+)
DBA/2J	MGP_DBA2J_G0015900	protein coding gene	Chr1:55986397-56055348 (+)
FVB/NJ	MGP_FVBNJ_G0015902	protein coding gene	Chr1:55329049-55373924 (+)
LP/J	MGP_LPJ_G0015972	protein coding gene	Chr1:59516197-59570524 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0015922	protein coding gene	Chr1:65793729-65853002 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0016495	protein coding gene	Chr1:58076028-58122118 (+)
PWK/PhJ	MGP_PWKPhJ_G0015195	protein coding gene	Chr1:55292142-55405453 (+)
SPRET/EiJ	MGP_SPRETEiJ_G0014975	protein coding gene	Chr1:57566170-57632221 (+)
WSB/EiJ	MGP_WSBEiJ_G0015472	protein coding gene	Chr1:57775021-57823823 (+)

Homology

• Human Ortholog
  CFLAR, CASP8 and FADD like apoptosis regulator

• Vertebrate Orthologs
  3

Vertebrate Orthology Source

Alliance of Genome Resources

• Human Ortholog
  CFLAR, CASP8 and FADD like apoptosis regulator
  
• Synonyms
  CASH, CASP8AP1, Casper, c-FLIP, cFLIP, c-FLIPL, c-FLIPR, c-FLIPS, CLARP, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT
• Links
  HGNC:1876

  NCBI Gene ID: 8837

  neXtProt AC: NX_O15519

  UniProt: O15519

  
  
• Chr Location
  2q33.1; chr2:201116154-201176687 (+)  GRCh38

---

• MGI Vertebrate Homology
  Cflar stringent orthology
  1 human;1 mouse;1 rat;1 zebrafish
  
• HCOP
  vertebrate homology predictions: CFLAR
• Gene Tree
  Cflar
  

Mutations,
Alleles, and
Phenotypes

• Phenotype Summary
  39 phenotypes from 5 alleles in 4 genetic backgrounds
  1 phenotype from multigenic genotypes
  48 phenotype references
  

• All Mutations and Alleles
  13
• Chemically induced (other)
  1
• Endonuclease-mediated
  2
• Gene trapped
  1
• Radiation induced
  1
• Spontaneous
  1
• Targeted
  7
• Genomic Mutations
  2 involving Cflar
• Incidental Mutations
  APF
• Find Mice (IMSR)
  32 strains or lines available
• Comparison Matrix
  Gene Expression + Phenotype

Homozygous mutation of this gene results in embryonic lethality by E10.5. Mutant embryos exhibit cardiac developmental abnormalities and pooling of blood in the head and abdominal regions.

Gene Ontology
(GO)
Classifications

• All GO Annotations
  60
• GO References
  12

Expression

• Assay Results
  959
• Tissues
  64
• cDNA Data
  20
• Literature Summary
  15

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

• Other Mouse Links
  Allen Institute
  GEO
  Expression Atlas
• Other Vertebrate Links
  Xenbase cflar
  ZFIN cflara

Sequences &
Gene Models

• All Sequences
  94
• RefSeq
  30
• UniProt
  5
• CCDS
  CCDS14978.1, CCDS35582.1

• Ensembl
  ENSMUSG00000026031 (Evidence)
• NCBI Gene
  12633

Representative Sequences			Length	Strain/Species
genomic	ENSMUSG00000026031	Ensembl Gene Model | MGI Sequence Detail	47377	C57BL/6J
transcript	ENSMUST00000097722	Ensembl | MGI Sequence Detail	1771	Not Applicable
polypeptide	ENSMUSP00000095329	Ensembl | MGI Sequence Detail	484	Not Applicable

Protein
Information

• UniProt
  5 Sequences
• Protein Ontology
  PR:000002282 CASP8 and FADD-like apoptosis regulator
  (term hierarchy)
• InterPro Domains
  IPR051290 CASP8 and FADD-like apoptosis regulator
  IPR029030 Caspase-like domain superfamily
  IPR001875 Death effector domain
  IPR011029 Death-like domain superfamily
  IPR015917 Peptidase C14A, caspase catalytic domain
  IPR011600 Peptidase C14, caspase domain
  IPR001309 Peptidase C14, p20 domain

Molecular
Reagents

• All nucleic 23

  Genomic 1

  cDNA 22

  
  

  Microarray probesets 10

Other
Accession IDs

MGI:1096876, MGI:1916820, MGI:1925024, MGI:2138307, MGI:2138466

References

• Summaries
  All 131

  Developmental Gene Expression 15

  Gene Ontology 12

  Phenotypes 48
• Earliest
  J:134667 Roderick TH, et al., Two radiation-induced chromosomal inversions in mice (Mus musculus). Proc Natl Acad Sci U S A. 1970 Oct;67(2):961-7
• Latest
  J:351982 Yang X, et al., cFLIP in the molecular regulation of astroglia-driven neuroinflammation in experimental glaucoma. J Neuroinflammation. 2024 Jun 1;21(1):145

TSS for Cflar:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr6785	Chr1:58750621-58750632 (+)	-40 bp
Tssr6786	Chr1:58750647-58750682 (+)	-2 bp
Tssr6787	Chr1:58751220-58751248 (+)	567 bp
Tssr6788	Chr1:58751555-58751593 (+)	907 bp
Tssr6789	Chr1:58752363-58752376 (+)	1,703 bp
Tssr6790	Chr1:58752400-58752473 (+)	1,770 bp
Tssr6791	Chr1:58752479-58752491 (+)	1,818 bp
Tssr6792	Chr1:58752537-58752540 (+)	1,872 bp
Tssr6793	Chr1:58752605-58752616 (+)	1,944 bp
Tssr6794	Chr1:58753323-58753359 (+)	2,674 bp
Tssr6795	Chr1:58753475-58753482 (+)	2,812 bp
Tssr6796	Chr1:58753803-58753816 (+)	3,143 bp
Tssr6797	Chr1:58756001-58756004 (+)	5,336 bp
Tssr6798	Chr1:58756609-58756628 (+)	5,952 bp
Tssr6799	Chr1:58761888-58761906 (+)	11,230 bp
Tssr6800	Chr1:58765175-58765222 (+)	14,532 bp
Tssr6801	Chr1:58765223-58765234 (+)	14,562 bp
Tssr6802	Chr1:58765254-58765265 (+)	14,593 bp
Tssr6803	Chr1:58765323-58765337 (+)	14,663 bp
Tssr6804	Chr1:58765355-58765381 (+)	14,701 bp
Tssr6805	Chr1:58766409-58766420 (+)	15,748 bp
Tssr6806	Chr1:58767774-58767785 (+)	17,113 bp
Tssr6807	Chr1:58767811-58767857 (+)	17,167 bp
Tssr6808	Chr1:58767872-58767883 (+)	17,211 bp
Tssr6809	Chr1:58768716-58768718 (+)	18,050 bp
Tssr6810	Chr1:58777591-58777611 (+)	26,934 bp
Tssr6811	Chr1:58777811-58777832 (+)	27,155 bp
Tssr6812	Chr1:58778133-58778156 (+)	27,478 bp
Tssr6813	Chr1:58782706-58782709 (+)	32,041 bp
Tssr6814	Chr1:58784303-58784311 (+)	33,640 bp
Tssr6815	Chr1:58788552-58788578 (+)	37,898 bp
Tssr6816	Chr1:58792853-58792858 (+)	42,189 bp
Tssr6817	Chr1:58793233-58793280 (+)	42,590 bp
Tssr6818	Chr1:58793901-58793917 (+)	43,242 bp
Tssr6819	Chr1:58793961-58793980 (+)	43,304 bp
Tssr6820	Chr1:58794040-58794055 (+)	43,381 bp
Tssr6821	Chr1:58794074-58794085 (+)	43,413 bp
Tssr6822	Chr1:58794141-58794152 (+)	43,480 bp
Tssr6823	Chr1:58794240-58794251 (+)	43,579 bp
Tssr6824	Chr1:58794274-58794286 (+)	43,613 bp
Tssr6825	Chr1:58794381-58794393 (+)	43,720 bp
Tssr6826	Chr1:58794482-58794499 (+)	43,824 bp
Tssr6827	Chr1:58795116-58795127 (+)	44,455 bp
Tssr6828	Chr1:58795425-58795440 (+)	44,766 bp
Tssr6829	Chr1:58795457-58795472 (+)	44,798 bp
Tssr6830	Chr1:58795504-58795560 (+)	44,865 bp
Tssr6831	Chr1:58795965-58795982 (+)	45,307 bp
Tssr6832	Chr1:58797538-58797562 (+)	46,883 bp