Myh4 Gene Detail

Summary

• Symbol
  Myh4
• Name
  myosin, heavy polypeptide 4, skeletal muscle
• Synonyms
  MHC2B, Minimsc, Minmus, MM, MYH-2B, MyHC-IIb, Myhsf

• Feature Type
  protein coding gene
• IDs
  MGI:1339713
  NCBI Gene: 17884
• Alliance
  gene page
• Transcription Start Sites
  61 TSS

Location &
Maps

• Sequence Map
  Chr11:67128855-67151272 bp, + strand

  From Ensembl annotation of GRCm39
  

• View this region in JBrowse
  

• Genome Browsers
  Ensembl | UCSC | NCBI

• Genetic Map
  Chromosome 11, 40.59 cM
• Mapping Data
  9 experiments

Strain
Comparison

• SNPs within 2kb
  780 from dbSNP Build 142
• Strain Annotations
  14
• RFLP
  6

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_1339713	protein coding gene	Chr11:67128638-67151273 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0018488	protein coding gene	Chr11:67533505-67555896 (+)
A/J	MGP_AJ_G0018457	protein coding gene	Chr11:65014933-65021971 (+)
AKR/J	MGP_AKRJ_G0018426	protein coding gene	Chr11:66944207-66968781 (+)
BALB/cJ	MGP_BALBcJ_G0018428	protein coding gene	Chr11:65278610-65301029 (+)
C3H/HeJ	MGP_C3HHeJ_G0018241	protein coding gene	Chr11:66956014-66979850 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0018880	protein coding gene	Chr11:69580151-69606375 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0016524	protein coding gene	Chr11:61954043-61976962 (+)
CAST/EiJ	MGP_CASTEiJ_G0017797	protein coding gene	Chr11:67178625-67201078 (+)
CBA/J	MGP_CBAJ_G0018215	protein coding gene	Chr11:72648011-72676873 (+)
DBA/2J	MGP_DBA2J_G0018324	protein coding gene	Chr11:64604056-64628426 (+)
FVB/NJ	MGP_FVBNJ_G0018314	protein coding gene	Chr11:64208342-64232077 (+)
LP/J	no annotation
NOD/ShiLtJ	no annotation
NZO/HlLtJ	MGP_NZOHlLtJ_G0018921	protein coding gene	Chr11:67184678-67210325 (+)
PWK/PhJ	no annotation
SPRET/EiJ	no annotation
WSB/EiJ	MGP_WSBEiJ_G0017850	protein coding gene	Chr11:66793831-66817525 (+)

Homology

• Human Ortholog
  MYH4, myosin heavy chain 4

• Vertebrate Orthologs
  9

Vertebrate Orthology Source

Alliance of Genome Resources

• Human Ortholog
  MYH4, myosin heavy chain 4
  
• Synonyms
  MYH2B, MyHC-2B, MyHC-IIb
• Links
  HGNC:7574

  NCBI Gene ID: 4622

  neXtProt AC: NX_Q9Y623

  UniProt: Q9Y623

  
  
• Chr Location
  17p13.1; chr17:10443263-10469559 (-)  GRCh38

---

• MGI Vertebrate Homology
  Myh4 stringent orthology
  1 human;1 mouse;1 rat;7 zebrafish
  
• HCOP
  vertebrate homology predictions: MYH4
• Gene Tree
  Myh4
  

Mutations,
Alleles, and
Phenotypes

• Phenotype Summary
  18 phenotypes from 2 alleles in 2 genetic backgrounds
  1 images
  19 phenotype references
  

• All Mutations and Alleles
  14
• Chemically induced (ENU)
  1
• Chemically induced (other)
  1
• Endonuclease-mediated
  4
• QTL
  3
• Targeted
  5
• Genomic Mutations
  4 involving Myh4
• Incidental Mutations
  Mutagenetix , APF , CvDC
• Find Mice (IMSR)
  95 strains or lines available
• Comparison Matrix
  Gene Expression + Phenotype

Homozygotes for a targeted null mutation exhibit decreased growth and muscle defects including reduced muscle mass, muscle fiber loss, compensatory fiber hypertrophy, and impaired strength.

Gene Ontology
(GO)
Classifications

• All GO Annotations
  17
• GO References
  7

Expression

• Assay Results
  1044
• Tissues
  59
• cDNA Data
  14
• Literature Summary
  60

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

• Other Mouse Links
  Allen Institute
  GEO
  Expression Atlas
• Other Vertebrate Links
  GEISHA MYH1C, MYH1F
  Xenbase myh4

Sequences &
Gene Models

• All Sequences
  60
• RefSeq
  2
• UniProt
  6
• CCDS
  CCDS24856.1

• Ensembl
  ENSMUSG00000057003 (Evidence)
• NCBI Gene
  17884

Representative Sequences			Length	Strain/Species
genomic	ENSMUSG00000057003	Ensembl Gene Model | MGI Sequence Detail	22418	C57BL/6J
transcript	ENSMUST00000018632	Ensembl | MGI Sequence Detail	6016	Not Applicable
polypeptide	ENSMUSP00000018632	Ensembl | MGI Sequence Detail	1939	Not Applicable

Protein
Information

• UniProt
  6 Sequences
• Protein Ontology
  PR:000010828 myosin-4
  (term hierarchy)
• PDB
  7NEP, 7QIN, 7QIO
• InterPro Domains
  IPR014751 DNA repair protein XRCC4-like, C-terminal
  IPR036961 Kinesin motor domain superfamily
  IPR001609 Myosin head, motor domain
  IPR004009 Myosin, N-terminal, SH3-like
  IPR008989 Myosin S1 fragment, N-terminal
  IPR002928 Myosin tail
  IPR027417 P-loop containing nucleoside triphosphate hydrolase
• GlyGen
  Q5SX39 1 site, 1 O-linked glycan (1 site)

Molecular
Reagents

• All nucleic 41

  Genomic 9

  cDNA 15

  Primer pair 15

  Other 2

  
  

  Microarray probesets 4

Other
Accession IDs

MGI:1261431, MGI:2144213, MGI:4420408

References

• Summaries
  All 124

  Developmental Gene Expression 60

  Gene Ontology 7

  Phenotypes 19
• Earliest
  J:137335 Roderick TH, Chromosomal inversions in studies of mammalian mutagenesis. Genetics. 1979 May;92(1 Pt 1 Suppl):s121-6
• Latest
  J:347774 Kolonay DW, et al., Temporal regulation of the Mediator complex during muscle proliferation, differentiation, regeneration, aging, and disease. Front Cell Dev Biol. 2024;12:1331563

TSS for Myh4:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr102164	Chr11:67127011-67127022 (+)	-1,838 bp
Tssr102165	Chr11:67127030-67127037 (+)	-1,821 bp
Tssr102166	Chr11:67128590-67128602 (+)	-259 bp
Tssr102167	Chr11:67128626-67128644 (+)	-220 bp
Tssr102168	Chr11:67128678-67128690 (+)	-171 bp
Tssr102169	Chr11:67128843-67128873 (+)	3 bp
Tssr102170	Chr11:67131383-67131431 (+)	2,552 bp
Tssr102171	Chr11:67131737-67131772 (+)	2,900 bp
Tssr102172	Chr11:67133635-67133644 (+)	4,785 bp
Tssr102173	Chr11:67133652-67133663 (+)	4,803 bp
Tssr102174	Chr11:67134049-67134052 (+)	5,196 bp
Tssr102175	Chr11:67134085-67134115 (+)	5,245 bp
Tssr102176	Chr11:67134249-67134270 (+)	5,405 bp
Tssr102177	Chr11:67134275-67134289 (+)	5,427 bp
Tssr102178	Chr11:67135463-67135475 (+)	6,614 bp
Tssr102179	Chr11:67136257-67136294 (+)	7,421 bp
Tssr102180	Chr11:67136595-67136609 (+)	7,747 bp
Tssr102181	Chr11:67139387-67139393 (+)	10,535 bp
Tssr3525	Chr11:67139403-67139414 (+)	10,554 bp
Tssr102182	Chr11:67139508-67139519 (+)	10,659 bp
Tssr102183	Chr11:67140083-67140092 (+)	11,233 bp
Tssr102184	Chr11:67140651-67140659 (+)	11,800 bp
Tssr102185	Chr11:67140681-67140692 (+)	11,832 bp
Tssr102186	Chr11:67140830-67140839 (+)	11,980 bp
Tssr102187	Chr11:67140865-67140877 (+)	12,016 bp
Tssr102188	Chr11:67141139-67141164 (+)	12,297 bp
Tssr3526	Chr11:67141189-67141203 (+)	12,341 bp
Tssr102189	Chr11:67141332-67141352 (+)	12,487 bp
Tssr102190	Chr11:67141359-67141368 (+)	12,509 bp
Tssr102191	Chr11:67141370-67141389 (+)	12,525 bp
Tssr102192	Chr11:67141390-67141395 (+)	12,538 bp
Tssr102193	Chr11:67141431-67141438 (+)	12,580 bp
Tssr102194	Chr11:67141573-67141580 (+)	12,722 bp
Tssr102195	Chr11:67141653-67141663 (+)	12,803 bp
Tssr102196	Chr11:67141691-67141702 (+)	12,842 bp
Tssr102197	Chr11:67141719-67141746 (+)	12,878 bp
Tssr102198	Chr11:67141797-67141807 (+)	12,947 bp
Tssr102199	Chr11:67142525-67142561 (+)	13,688 bp
Tssr102200	Chr11:67142676-67142712 (+)	13,839 bp
Tssr102201	Chr11:67144301-67144344 (+)	15,468 bp
Tssr102202	Chr11:67144823-67144833 (+)	15,973 bp
Tssr102203	Chr11:67144883-67144896 (+)	16,035 bp
Tssr102204	Chr11:67145281-67145292 (+)	16,432 bp
Tssr102205	Chr11:67145499-67145509 (+)	16,649 bp
Tssr3528	Chr11:67145559-67145569 (+)	16,709 bp
Tssr102206	Chr11:67145603-67145613 (+)	16,753 bp
Tssr3529	Chr11:67145615-67145625 (+)	16,765 bp
Tssr102207	Chr11:67146247-67146266 (+)	17,402 bp
Tssr102208	Chr11:67146417-67146429 (+)	17,568 bp
Tssr102209	Chr11:67146452-67146468 (+)	17,605 bp
Tssr102210	Chr11:67146470-67146479 (+)	17,620 bp
Tssr102211	Chr11:67146480-67146496 (+)	17,633 bp
Tssr102212	Chr11:67146502-67146514 (+)	17,653 bp
Tssr102213	Chr11:67146654-67146666 (+)	17,805 bp
Tssr102214	Chr11:67147010-67147017 (+)	18,159 bp
Tssr102215	Chr11:67147024-67147041 (+)	18,178 bp
Tssr102216	Chr11:67147068-67147077 (+)	18,218 bp
Tssr102217	Chr11:67147222-67147231 (+)	18,372 bp
Tssr102218	Chr11:67147933-67147942 (+)	19,083 bp
Tssr102219	Chr11:67148020-67148037 (+)	19,174 bp
Tssr102220	Chr11:67148059-67148075 (+)	19,212 bp