Cd274 Gene Detail

Summary

• Symbol
  Cd274
• Name
  CD274 antigen
• Synonyms
  B7-H1, Pdcd1lg1, PD-L1

• Feature Type
  protein coding gene
• IDs
  MGI:1926446
  NCBI Gene: 60533
• Alliance
  gene page
• Transcription Start Sites
  74 TSS
• Candidate for QTL
  1 QTL

Location &
Maps

• Sequence Map
  Chr19:29344855-29365495 bp, + strand

  From Ensembl annotation of GRCm39
  

• View this region in JBrowse
  

• Genome Browsers
  Ensembl | UCSC | NCBI

• Genetic Map
  Chromosome 19, 23.88 cM, cytoband C2
• Mapping Data
  2 experiments

Strain
Comparison

• SNPs within 2kb
  731 from dbSNP Build 142
• Strain Annotations
  18

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_1926446	protein coding gene	Chr19:29339428-29365495 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0025022	protein coding gene	Chr19:27821779-27846578 (+)
A/J	MGP_AJ_G0024999	protein coding gene	Chr19:26420253-26441355 (+)
AKR/J	MGP_AKRJ_G0024967	protein coding gene	Chr19:27378703-27400807 (+)
BALB/cJ	MGP_BALBcJ_G0024998	protein coding gene	Chr19:26469693-26490495 (+)
C3H/HeJ	MGP_C3HHeJ_G0024754	protein coding gene	Chr19:27355110-27377246 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0025439	protein coding gene	Chr19:28595293-28617586 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0022858	protein coding gene	Chr19:26391525-26411837 (+)
CAST/EiJ	MGP_CASTEiJ_G0024224	protein coding gene	Chr19:27183986-27220273 (+)
CBA/J	MGP_CBAJ_G0024732	protein coding gene	Chr19:29943620-29966583 (+)
DBA/2J	MGP_DBA2J_G0024864	protein coding gene	Chr19:26378127-26399100 (+)
FVB/NJ	MGP_FVBNJ_G0024826	protein coding gene	Chr19:26235082-26255903 (+)
LP/J	MGP_LPJ_G0024949	protein coding gene	Chr19:27824697-27846606 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0024853	protein coding gene	Chr19:29885016-29908371 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0025494	protein coding gene	Chr19:27396928-27419353 (+)
PWK/PhJ	MGP_PWKPhJ_G0023972	protein coding gene	Chr19:26051593-26073656 (+)
SPRET/EiJ	MGP_SPRETEiJ_G0023776	protein coding gene	Chr19:26671792-26694642 (+)
WSB/EiJ	MGP_WSBEiJ_G0024291	protein coding gene	Chr19:27445681-27470745 (+)

Homology

• Human Ortholog
  CD274, CD274 molecule

• Vertebrate Orthologs
  3

Vertebrate Orthology Source

Alliance of Genome Resources

• Human Ortholog
  CD274, CD274 molecule
  
• Synonyms
  B7-H, B7H1, hPD-L1, PDCD1L1, PDCD1LG1, PD-L1, PDL1
• Links
  HGNC:17635

  NCBI Gene ID: 29126

  neXtProt AC: NX_Q9NZQ7

  UniProt: Q9NZQ7

  
  
• Chr Location
  9p24.1; chr9:5450503-5470566 (+)  GRCh38

---

• MGI Vertebrate Homology
  Cd274 stringent orthology
  1 human;1 mouse;1 rat;1 zebrafish
  
• HCOP
  vertebrate homology predictions: CD274
• Gene Tree
  Cd274
  

Mutations,
Alleles, and
Phenotypes

• Phenotype Summary
  43 phenotypes from 5 alleles in 9 genetic backgrounds
  7 phenotypes from multigenic genotypes
  155 phenotype references
  

• All Mutations and Alleles
  34
• Endonuclease-mediated
  14
• Targeted
  19
• Transgenic
  1
• Genomic Mutations
  3 involving Cd274
• Incidental Mutations
  Mutagenetix , APF
• Find Mice (IMSR)
  70 strains or lines available
• Comparison Matrix
  Gene Expression + Phenotype

Mice homozygous for a knock-out allele exhibit altered susceptibility to experimental autoimmune encephalomyelitis, induced arthritis, nerve injury, autoimmune diabetes, bacterial infection, viral infection, and parasitic infection due to abnormal T cellmorphology and physiology.

Gene Ontology
(GO)
Classifications

• All GO Annotations
  64
• GO References
  13

Expression

• Assay Results
  717
• Tissues
  4
• cDNA Data
  17
• Literature Summary
  5

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

• Other Mouse Links
  Allen Institute
  GEO
  Expression Atlas

Sequences &
Gene Models

• All Sequences
  37
• RefSeq
  6
• UniProt
  2
• CCDS
  CCDS29735.1

• Ensembl
  ENSMUSG00000016496 (Evidence)
• NCBI Gene
  60533

Representative Sequences			Length	Strain/Species
genomic	ENSMUSG00000016496	Ensembl Gene Model | MGI Sequence Detail	20641	C57BL/6J
transcript	ENSMUST00000016640	Ensembl | MGI Sequence Detail	3622	Not Applicable
polypeptide	ENSMUSP00000016640	Ensembl | MGI Sequence Detail	290	Not Applicable

Protein
Information

• UniProt
  2 Sequences
• Protein Ontology
  PR:000001543 programmed cell death 1 ligand 1
  (term hierarchy)
• PDB
  6SRU
• InterPro Domains
  IPR013162 CD80-like, immunoglobulin C2-set
  IPR007110 Immunoglobulin-like domain
  IPR036179 Immunoglobulin-like domain superfamily
  IPR013783 Immunoglobulin-like fold
  IPR003599 Immunoglobulin subtype
  IPR013106 Immunoglobulin V-set domain
  IPR051713 T-cell Activation and Immune Regulation Protein
• GlyGen
  Q9EP73 6 sites, 4 N-linked glycans (4 sites)

Molecular
Reagents

• All nucleic 20

  cDNA 17

  Primer pair 3

  
  

  Microarray probesets 3

References

• Summaries
  All 307

  Developmental Gene Expression 5

  Gene Ontology 13

  Phenotypes 155
• Earliest
  J:64991 Freeman GJ, et al., Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med. 2000 Oct 2;192(7):1027-34
• Latest
  J:359311 Lin F, et al., Multimodal targeting chimeras enable integrated immunotherapy leveraging tumor-immune microenvironment. Cell. 2024 Nov 2;

TSS for Cd274:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr155806	Chr19:29343956-29343968 (+)	-893 bp
Tssr155807	Chr19:29344528-29344576 (+)	-303 bp
Tssr155808	Chr19:29344592-29344599 (+)	-259 bp
Tssr155809	Chr19:29344847-29344868 (+)	3 bp
Tssr155810	Chr19:29346238-29346257 (+)	1,393 bp
Tssr155811	Chr19:29346450-29346461 (+)	1,601 bp
Tssr155812	Chr19:29347626-29347658 (+)	2,787 bp
Tssr155813	Chr19:29347661-29347674 (+)	2,813 bp
Tssr155814	Chr19:29347738-29347777 (+)	2,903 bp
Tssr155815	Chr19:29349715-29349724 (+)	4,865 bp
Tssr155816	Chr19:29349745-29349759 (+)	4,897 bp
Tssr155817	Chr19:29349857-29349898 (+)	5,023 bp
Tssr155818	Chr19:29350951-29350969 (+)	6,105 bp
Tssr155819	Chr19:29350991-29351003 (+)	6,142 bp
Tssr155820	Chr19:29351033-29351051 (+)	6,187 bp
Tssr155821	Chr19:29351115-29351131 (+)	6,268 bp
Tssr155822	Chr19:29351147-29351162 (+)	6,300 bp
Tssr155823	Chr19:29351181-29351213 (+)	6,342 bp
Tssr155824	Chr19:29351222-29351249 (+)	6,381 bp
Tssr155825	Chr19:29351761-29351783 (+)	6,917 bp
Tssr155826	Chr19:29353110-29353130 (+)	8,265 bp
Tssr155827	Chr19:29353568-29353578 (+)	8,718 bp
Tssr155828	Chr19:29354056-29354070 (+)	9,208 bp
Tssr155829	Chr19:29354474-29354487 (+)	9,626 bp
Tssr155830	Chr19:29354650-29354661 (+)	9,801 bp
Tssr155831	Chr19:29354729-29354761 (+)	9,890 bp
Tssr155832	Chr19:29354836-29354849 (+)	9,988 bp
Tssr155833	Chr19:29355190-29355211 (+)	10,346 bp
Tssr155834	Chr19:29355314-29355327 (+)	10,466 bp
Tssr155835	Chr19:29355336-29355348 (+)	10,487 bp
Tssr155836	Chr19:29355414-29355581 (+)	10,643 bp
Tssr155837	Chr19:29355582-29355651 (+)	10,762 bp
Tssr155838	Chr19:29356504-29356523 (+)	11,659 bp
Tssr155839	Chr19:29357676-29357687 (+)	12,827 bp
Tssr155840	Chr19:29357771-29357806 (+)	12,934 bp
Tssr155841	Chr19:29357825-29357849 (+)	12,982 bp
Tssr155842	Chr19:29357863-29357890 (+)	13,022 bp
Tssr155843	Chr19:29357896-29357909 (+)	13,048 bp
Tssr155844	Chr19:29357923-29357934 (+)	13,074 bp
Tssr155845	Chr19:29357937-29357950 (+)	13,089 bp
Tssr155846	Chr19:29357951-29357964 (+)	13,103 bp
Tssr155847	Chr19:29358716-29358750 (+)	13,878 bp
Tssr155848	Chr19:29359898-29359907 (+)	15,048 bp
Tssr155849	Chr19:29359914-29359933 (+)	15,069 bp
Tssr155850	Chr19:29360703-29360708 (+)	15,851 bp
Tssr155851	Chr19:29361013-29361032 (+)	16,168 bp
Tssr155852	Chr19:29361048-29361078 (+)	16,208 bp
Tssr155853	Chr19:29361197-29361214 (+)	16,351 bp
Tssr155854	Chr19:29361491-29361522 (+)	16,652 bp
Tssr155855	Chr19:29361653-29361663 (+)	16,803 bp
Tssr155856	Chr19:29363014-29363025 (+)	18,165 bp
Tssr155857	Chr19:29363473-29363484 (+)	18,624 bp
Tssr155858	Chr19:29363713-29363725 (+)	18,864 bp
Tssr155859	Chr19:29363738-29363749 (+)	18,889 bp
Tssr155860	Chr19:29363762-29363776 (+)	18,914 bp
Tssr155861	Chr19:29363779-29363784 (+)	18,927 bp
Tssr155862	Chr19:29363888-29363916 (+)	19,047 bp
Tssr155863	Chr19:29363927-29363952 (+)	19,085 bp
Tssr155864	Chr19:29364009-29364049 (+)	19,174 bp
Tssr155865	Chr19:29364058-29364108 (+)	19,228 bp
Tssr155866	Chr19:29364132-29364153 (+)	19,288 bp
Tssr155867	Chr19:29364170-29364189 (+)	19,325 bp
Tssr155868	Chr19:29364227-29364240 (+)	19,379 bp
Tssr155869	Chr19:29364262-29364293 (+)	19,423 bp
Tssr155870	Chr19:29364294-29364309 (+)	19,447 bp
Tssr155871	Chr19:29364311-29364314 (+)	19,458 bp
Tssr155872	Chr19:29364324-29364383 (+)	19,499 bp
Tssr155873	Chr19:29364384-29364393 (+)	19,534 bp
Tssr155874	Chr19:29364395-29364406 (+)	19,546 bp
Tssr155875	Chr19:29364517-29364548 (+)	19,678 bp
Tssr155876	Chr19:29364652-29364672 (+)	19,807 bp
Tssr155877	Chr19:29364840-29364853 (+)	19,992 bp
Tssr155878	Chr19:29365016-29365023 (+)	20,165 bp
Tssr155879	Chr19:29365234-29365245 (+)	20,385 bp

Cd274 is Candidate Gene for:

QTL	Genetic Location*	Genome Location (GRCm39)	Reference	QTL Note
Tlyr1	Chr19, 21.34 cM	Chr19:20539528-25313796	J:168370	Three overlapping segments of the Tlyr1 locus resided in three SEG/Pas subcongenic strains. One of these regions proved informative to suggest that Cd274 may be a candidate for the Tlyr1 locus.

*cM position of peak correlated region/marker