Tg(CAG-dre)1Afst
Transgene Detail

Summary

• Symbol: Tg(CAG-dre)1Afst
• Name: transgene insertion 1, A Francis Stewart
• MGI ID: MGI:4367446
• Synonyms: CAG-Dre, CAGGs-Dre
• Transgene: Tg(CAG-dre)1Afst Location: unknown
• Alliance: Tg(CAG-dre)1Afst page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:154134
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(CAG-dre)1Afst expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: A plasmid in which the CAG (CAGGS) promoter drives the bacteriophage D6 site-specific recombinase (dre). This is followed by an IRES-puromycin sequence for selection. The linearized construct was electroporated into R1 ES cells, and single-copy clones were injected into 8-cell embryos. Germline transmission was obtained for one of these clones. (J:154134)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:154134 Anastassiadis K, et al., Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice. Dis Model Mech. 2009 Sep-Oct;2(9-10):508-15
• All: 24 reference(s)