Cd28 MGI Mouse Gene Detail - MGI:88327

Symbol

Cd28
Name

CD28 antigen

Feature Type

protein coding gene
IDs

MGI:88327
NCBI Gene: 12487
Alliance

gene page
Transcription Start Sites

7 TSS

Sequence Map

Chr1:60785547-60812521 bp, + strand
From NCBI annotation of GRCm39
View this region in JBrowse
Genome Browsers

Ensembl | UCSC | NCBI

Genetic Map

Chromosome 1, 30.52 cM, cytoband C1-C3
Mapping Data

17 experiments

SNPs within 2kb

208 from dbSNP Build 142
Strain Annotations

18
RFLP

2

For selected strains:

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_88327	protein coding gene	Chr1:60755959-60812521 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0016079	protein coding gene	Chr1:60803491-60854866 (+)
A/J	MGP_AJ_G0016062	protein coding gene	Chr1:58639659-58690015 (+)
AKR/J	MGP_AKRJ_G0016018	protein coding gene	Chr1:60297644-60346809 (+)
BALB/cJ	MGP_BALBcJ_G0016017	protein coding gene	Chr1:58675762-58725006 (+)
C3H/HeJ	MGP_C3HHeJ_G0015849	protein coding gene	Chr1:60535151-60585552 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0016470	protein coding gene	Chr1:63157518-63216713 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0014191	protein coding gene	Chr1:54842110-54898561 (+)
CAST/EiJ	MGP_CASTEiJ_G0015431	protein coding gene	Chr1:59741836-59792912 (+)
CBA/J	MGP_CBAJ_G0015821	protein coding gene	Chr1:65147627-65203584 (+)
DBA/2J	MGP_DBA2J_G0015922	protein coding gene	Chr1:58074563-58124328 (+)
FVB/NJ	MGP_FVBNJ_G0015924	protein coding gene	Chr1:57362133-57413296 (+)
LP/J	MGP_LPJ_G0015994	protein coding gene	Chr1:61675563-61727867 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0015944	protein coding gene	Chr1:68044546-68095668 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0016517	protein coding gene	Chr1:60223791-60275493 (+)
PWK/PhJ	MGP_PWKPhJ_G0015218	protein coding gene	Chr1:57415476-57466457 (+)
SPRET/EiJ	MGP_SPRETEiJ_G0014998	protein coding gene	Chr1:59725191-59779915 (+)
WSB/EiJ	MGP_WSBEiJ_G0015494	protein coding gene	Chr1:59914736-59964745 (+)

Human Ortholog

CD28, CD28 molecule

Vertebrate Orthologs

3

Vertebrate Orthology Source

Alliance of Genome Resources

Human Ortholog

CD28, CD28 molecule
Synonyms

IMD123, Tp44
Links

HGNC:1653

NCBI Gene ID: 940

neXtProt AC: NX_P10747

UniProt: P10747
Chr Location

2q33.2; chr2:203706475-203738912 (+) GRCh38

MGI Vertebrate Homology

Cd28 stringent orthology
1 human;1 mouse;1 rat;1 zebrafish
HCOP

vertebrate homology predictions: CD28
Gene Tree

Cd28

Diseases

2 with human CD28 associations

				Human Disease	Mouse Models
				multiple sclerosis IDs multiple sclerosis DOID:2377 ICD10CM:G35 ICD9CM:340 MESH:D009103 NCI:C3243 OMIM:612594 OMIM:612595 OMIM:612596 UMLS_CUI:C0026769
				type 1 diabetes mellitus IDs type 1 diabetes mellitus DOID:9744 ICD10CM:E10 KEGG:04940 MESH:D003922 NCI:C2986 OMIM:222100 UMLS_CUI:C0011854

Click on a disease name to see all genes associated with that disease.

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Phenotype Summary

55 phenotypes from 8 alleles in 15 genetic backgrounds
39 phenotypes from multigenic genotypes
3 images
350 phenotype references

Phenotype Overview

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene.

adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary system	embryo	endocrine/exocrine glands	growth/size/body	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	integument	immune system	limbs/digits/tail	liver/biliary system	mortality/aging	muscle	nervous system	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	taste/olfaction	neoplasm	vision/eye

Click cells to view annotations.

All Mutations and Alleles

22
Chemically induced (other)

1
Endonuclease-mediated

8
Radiation induced

1
Targeted

12
Genomic Mutations

2 involving Cd28
Incidental Mutations

Mutagenetix , CvDC
Find Mice (IMSR)

52 strains or lines available
Comparison Matrix

Gene Expression + Phenotype

Homozygous mutation of this gene results in impairment of some T cell responses and decreased basal immunoglobulin levels. Mutant animals have reduced T helper cell activity and impaired T cell response to lectins, but cytotoxic T cells can still be induced.

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All GO Annotations

56
GO References

23

Molecular Function

carbohydrate derivative binding	cytoskeletal protein binding	DNA binding	enzyme regulator	hydrolase	ligase	lipid binding	oxidoreductase	RNA binding	signaling receptor activity	signaling receptor binding	transcription	transferase	transporter

Biological Process

carbohydrate derivative metabolism	cell differentiation	cell population proliferation	cellular component organization	DNA-templated transcription	establishment of localization	homeostatic process	immune system process	lipid metabolic process	programmed cell death	protein metabolic process	response to stimulus	signaling	system development

Cellular Component

cell projection	cytoplasmic vesicle	cytoskeleton	cytosol	endoplasmic reticulum	endosome	extracellular region	Golgi apparatus	mitochondrion	membraneless organelle	nucleus	organelle envelope	organelle lumen	plasma membrane	protein-containing complex	synapse	vacuole

Click cells to view annotations.

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Expression Overview

GXD's primary emphasis is on endogenous gene expression during development. Click on grid cells to view annotations.

Blue cells = expressed in wild-type.
Gray triangles = other expression annotations only
(e.g. absence of expression or data from mutants).

early conceptus	embryo ectoderm	embryo endoderm	embryo mesoderm	embryo mesenchyme	extraembryonic component	alimentary system	auditory system	branchial arches	cardiovascular system	connective tissue	endocrine system	exocrine system	hemolymphoid system	integumental system	limbs	liver and biliary system	musculoskeletal system	nervous system	olfactory system	reproductive system	respiratory system	urinary system	visual system

Click cells to view annotations.

Assay Results

706
cDNA Data

35
Literature Summary

1

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

Other Mouse Links

Allen Institute

GEO

Expression Atlas
Other Vertebrate Links

Xenbase cd28

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All Sequences

39
RefSeq

4
UniProt

2
CCDS

CCDS14992.1

Ensembl

ENSMUSG00000026012 (Evidence)
NCBI Gene

12487

			Length	Strain/Species	Flank
genomic	12487	NCBI Gene Model \| MGI Sequence Detail	26975	C57BL/6J	± kb
transcript	NM_007642	RefSeq \| MGI Sequence Detail	4317	ZRU/MplStud
polypeptide	P31041	UniProt \| EBI \| MGI Sequence Detail	218	Not Applicable

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UniProt

2 Sequences
Protein Ontology

PR:000001841 T-cell-specific surface glycoprotein CD28

(term hierarchy)
PDB

2NAE
InterPro Domains

IPR040216 Cytotoxic T-lymphocyte protein 4/CD28

IPR036179 Immunoglobulin-like domain superfamily

IPR013783 Immunoglobulin-like fold

IPR013106 Immunoglobulin V-set domain

IPR008093 T cell antigen CD28
GlyGen

P31041 5 sites, 2 N-linked glycans (2 sites)

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All nucleic 38

cDNA 38

Microarray probesets 4

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MGD-MRK-1845

Summaries

All 502

Developmental Gene Expression 1

Gene Ontology 23

Phenotypes 350
Earliest

J:134667 Roderick TH, et al., Two radiation-induced chromosomal inversions in mice (Mus musculus). Proc Natl Acad Sci U S A. 1970 Oct;67(2):961-7
Latest

J:349941 Geels SN, et al., Interruption of the intratumor CD8(+) T cell:Treg crosstalk improves the efficacy of PD-1 immunotherapy. Cancer Cell. 2024 Jun 10;42(6):1051-1066.e7

TSS for Cd28:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr6938	Chr1:60785526-60785586 (+)	9 bp
Tssr6939	Chr1:60785588-60785636 (+)	65 bp
Tssr6940	Chr1:60785644-60785655 (+)	103 bp
Tssr6941	Chr1:60790875-60790878 (+)	5,330 bp
Tssr6942	Chr1:60794634-60794638 (+)	9,089 bp
Tssr6943	Chr1:60807367-60807371 (+)	21,822 bp
Tssr6944	Chr1:60810772-60810775 (+)	25,227 bp

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 11/12/2024 MGI 6.24