A 12.4-kb promoter fragment from the villin 1 gene drives the expression of cre recombinase cDNA with the entire metallothionein sequence and a poly(A) signal. Recombination occurs in villi and crypt cells of the small and large intestines, closely patterning the endogenous gene expression. Seven lines were generated. According to the corresponding author (J:78815), expression is generally continuous but a small amount of mosaicism is noted in the colon. Onset of transgene expression is at 12.5 dpc, which is delayed from the endogenous mouse Vil gene expression onset of 9.0 dpc. Line 997 contains 16 copies of the transgene inserted in chromosome 17 about 119 kb upstream of St6gal2 (chr17:55,466,255; NCBI37/mm9) with a 14.6 kb deletion at the integration site.
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