This transgene expresses a tamoxifen-inducible form of cre recombinase under the control of the tyrosinase enhancer and promoter, active in melanoblasts and melanocytes. The transgene inserted on the proximal end of chromosome 7 fused to a ~3 million base pair duplication from the proximal end of chromosome 2. This insertion also caused the deletion of some chromosome 7 loci. Specifically, the insertion occurred on chromosome 7 into exon 10 of the Nlrp12 locus (3,220,560; mouse mm10), causing deletion of centromeric sequences including the entire Speer9-ps1 and Au018091 loci as well as the remaining 3' Nlrp12 sequences. The duplicated chromosome 2 sequences (now fused to the transgene on chromosome 7) include the entire Fam171a1, Nmt2, Rpp38, Olah1, Meig1, and Dclre1c loci, as well as a portion of Suv39h2 lacking exons 1-2. Therefore, animals hemizygous for the Tg(Tyr-cre/ERT2)1Lru allele carries three copies of the duplicated chromosome 2 genes, but only one functional copy of the deleted chromosome 7 genes.
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