About   Help   FAQ
Tg(Sp7-tTA,tetO-EGFP/cre)1Amc - Limbs
Recombinase Activity Detail
Allele Information
Allele:
Tg(Sp7-tTA,tetO-EGFP/cre)1Amc
transgene insertion 1, Andrew P McMahon 		Driver: Sp7
Type: Transgenic (Inducible, Recombinase, Reporter, Transactivator)
Inducer: doxycycline/tetracycline
Synonym: osterix-Cre, Osx1-cre, Osx1-Cre, Osx1-GFP::Cre, Osx-Cre, Osx-Cre::GFP, Osx-gfp, The Sp7-Cre-EGFP
Molecular description: A tTA-regulated GFP::Cre construct, which has the tetracycline-regulated transactivator (tTA) cassette upstream of a tetracycline-responsive element (tetO)-controlled Enhanced Green Fluorescent Protein/cre fusion protein was used to modify a bacterial artificial chromosome (BAC) encoding exon 1 of the mouse Sp7 gene (formerly osterix or Osx1). An frt-flanked selection cassette following the tTA-regulated GFP::Cre was removed from correctly targeted bacteria via transient flpe expression. A 5.2 kb portion of the modified BAC (Osx1-GFP::Cre) was used for pronuclear injection into fertilized CD1 embryos.
Find mice (IMSR): Mouse Strains: 2 strains available       Cell Lines: 0 lines available
Additional Tissues: Tg(Sp7-tTA,tetO-EGFP/cre)1Amc activity also observed in:
mesenchyme, mouse-other, skeletal system
Tissue Information
Limbs Other recombinase alleles with activity in Limbs tissues:
Images
Drag images to compare to others or to data in the table below. Drag corners to resize images for more detail. Reset Images
J:114494 Fig. 2
Recombinase Activity
Click heading to re-sort table.
Assays Genotypic Background Result Notes  
References
All for this allele: 302 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory