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Tg(Sp7-tTA,tetO-EGFP/cre)1Amc - Mouse-other
Recombinase Activity Detail
Allele Information
Allele:
Tg(Sp7-tTA,tetO-EGFP/cre)1Amc
transgene insertion 1, Andrew P McMahon 		Driver: Sp7
Type: Transgenic (Inducible, Recombinase, Reporter, Transactivator)
Inducer: doxycycline/tetracycline
Synonym: osterix-Cre, Osx1-cre, Osx1-Cre, Osx1-GFP::Cre, Osx-Cre, Osx-Cre::GFP, Osx-gfp, The Sp7-Cre-EGFP
Molecular description: A tTA-regulated GFP::Cre construct, which has the tetracycline-regulated transactivator (tTA) cassette upstream of a tetracycline-responsive element (tetO)-controlled Enhanced Green Fluorescent Protein/cre fusion protein was used to modify a bacterial artificial chromosome (BAC) encoding exon 1 of the mouse Sp7 gene (formerly osterix or Osx1). An frt-flanked selection cassette following the tTA-regulated GFP::Cre was removed from correctly targeted bacteria via transient flpe expression. A 5.2 kb portion of the modified BAC (Osx1-GFP::Cre) was used for pronuclear injection into fertilized CD1 embryos.
Find mice (IMSR): Mouse Strains: 2 strains available       Cell Lines: 0 lines available
Additional Tissues: Tg(Sp7-tTA,tetO-EGFP/cre)1Amc activity also observed in:
limbs, mesenchyme, skeletal system
Tissue Information
Mouse-other Other recombinase alleles with activity in Mouse-other tissues:
Images
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J:114494 Fig. 2
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 302 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory