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Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo
transgene insertion 1, Andrew Wolfe 		Driver: Gnrh1
Type: Transgenic (Inducible, Inserted expressed sequence, Recombinase)
Inducer: doxycycline/tetracycline
Synonym: GnRH-CRETeR
Molecular description: A -3446 bp fragment of the mouse Gnrh1 promoter was fused upstream of cre recombinase. The proximal region of the Gnrh1 promoter was modified to insert two Tet operators (tetO2). In series, the transgene also contains the CMV promoter regulating the Tet repressor gene (tetR). The rabbit beta-globin intron II is inserted between the promoter and the gene to enhance expression of the tetR gene. In absence of doxycycline, the tet repressor protein binds to the tet operators, blocking transcription. Induction by doxycycline releases the repression, permitting transcription of cre recombinase. Four founders were generated and individually analyzed for function and specificity. A representative line no. 1 was created.
Find mice (IMSR): Mouse Strains: 0 strains available       Cell Lines: 0 lines available
Additional Tissues: Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo activity also observed in:
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Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory