Ascl1<tm1.1(cre/ERT2)Jejo> - Head Recombinase Activity Detail MGI Mouse

Ascl1^{tm1.1(cre/ERT2)Jejo} - Head
Recombinase Activity Detail

Allele Information | Tissue Information | Recombinase Activity | References

Allele Information

Allele:

Ascl1^{tm1.1(cre/ERT2)Jejo}
achaete-scute family bHLH transcription factor 1; targeted mutation 1.1, Jane Johnson

Driver: Ascl1
Type: Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Synonym:

Ascl1-CreER, Ascl1^CreERT2

Molecular description:

A targeting vector was designed to replace the entire coding region of the achaete-scute complex homolog 1 (Ascl1 or Mash1) with a CreERT2 fusion protein and a frt-flanked neomycin (neo) selection cassette. The construct was electroporated into 129S/SvEv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to C57BL/6 mice. These mice were then bred to mice containing Flp-recombinase to remove the Frt-flanked sequence. These mice were then backcrossed at least 5 generations to and outbred ICR strain generate a colony of Ascl1-CreERT2 mice.

Find mice (IMSR):

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Additional Tissues:

Ascl1^{tm1.1(cre/ERT2)Jejo} activity also observed in:
nervous system

Tissue Information

Head

Other recombinase alleles with activity in Head tissues:

7630403G23Rik^{Tg(Th-cre)1Tmd}, Abcg2^{tm1.1(cre/Esr1*)Jvbe}, Acan^{tm1(cre/ERT2)Crm}, Acsm4^tm1(cre)Hsak ...(more)

Recombinase Activity

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Assays Genotypic Background Result Notes

References

All for this allele:

75 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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