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Tg(Cspg4-cre/Esr1*)BAkik - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Tg(Cspg4-cre/Esr1*)BAkik
transgene insertion B, Akiko Nishiyama 		Driver: Cspg4
Type: Transgenic (Inducible, Recombinase)
Inducer: tamoxifen
Synonym: NG2-CreER, NG2-CreER, NG2creERB, NG2creERTM, NG2creERTM
Molecular description: The 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences), was modified by inserting a CreERTM fusion gene (cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand-binding domain) followed by a rabbit beta-globin polyA signal, into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent the translation from the BAC NG2 gene and to allow translation from the first ATG in the CreERTM sequence. The ~219 kb BAC sequence was linearized and microinjected into the pronucleus of fertilized oocytes from C57BL/6 or (C57BL/6 x SJL)F1 mice. Mice derived from line B (NG2creERB) had no background cre activity in the absence of tamoxifen, with cre activity specifically in NG2-expressing cells after tamoxifen injection. Transgene insertion occurred on Chr 14, causing a 628 bp deletion.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Tg(Cspg4-cre/Esr1*)BAkik activity also observed in:
Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Images
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J:162529 Fig. 4 J:232845 Full Image
Fig. Cspg4 adult Cerebral Cortex J:232845 Full image
Fig. Cspg4 adult Hipocampus J:232845 Full Image
Fig. Cspg4 adult Hypothalamus J:232845 Full Image
Fig. Cspg4 adult Medulla J:232845 Full Image
Fig. Cspg4 adult Midbrain J:232845 Full Image
Fig. Cspg4 adult Olfactory bulb J:232845 Full Image
Fig. Cspg4 adult Pons
J:232845 Full Image
Fig. Cspg4 adult SC cervical J:232845 Full Image
Fig. Cspg4 adult SC Lumbar J:232845 Full Image
Fig. Cspg4 adult SC Thoracic
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 140 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory