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Tg(Adipoq-cre)1Evdr - Reproductive System
Recombinase Activity Detail
Allele Information
Allele:
Tg(Adipoq-cre)1Evdr
transgene insertion 1, Evan Rosen 		Driver: Adipoq
Type: Transgenic (Recombinase)
Synonym: A-Cre, Adipoq-Cre, Adn-Cre, Tg(Adipoq-CreERT2)tm1Jgg
Molecular description: To generate the Adipoq-Cre BAC transgenic mice, the 245 kb RP23-90G21 bacterial artificial chromosome (BAC) containing at least five mouse genes was modified by replacing the starting ATG and 222 bp of the adiponectin gene with the starting ATG and coding sequence of a Cre recombinase gene. Founder line 1 was analyzed. These mice express Cre recombinase effectively in white adipose tissue (WAT) and brown adipose tissue (BAT), but not in macrophages (including adipose-tissue resident macrophages, alveolar macrophages, or thioglycollate-stimulated peritoneal macrophages). The investigator reports highly efficient Cre recombinase activity, with no ectopic expression. The transgene integrated on chromosome 9 between exons 6 and 7 of the Tbx18 locus, an embryonic development gene also associated with myocardial function. No genomic rearrangements were detected in the region of the integration site, although the insertion leads to reduced Tbx18 expression in hemizygous epididymal white adipose tissue. While the targeted locus amplification mapping predicted transgene-transgene fusions had occurred between different parts of the transgene, their ddPCR analysis identified the number of copies of intact Cre recombinase as one per genome.
Find mice (IMSR): Mouse Strains: 3 strains available       Cell Lines: 0 lines available
Additional Tissues: Tg(Adipoq-cre)1Evdr activity also observed in:
adipose tissue, alimentary system, embryo-other, endocrine system, integumental system, mesenchyme
Tissue Information
Reproductive System Other recombinase alleles with activity in Reproductive System tissues:
Images
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J:193672 Full Image
Fig. Adipoq Testis (WAT) P7
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 533 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory