A targeting vector was designed to insert a Cre/ERT2 fusion gene , an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the ets variant gene 1 locus (Etv1). This construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with white C57BL/6 mice (harboring the Tyrc-2J mutation) to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette.
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