Nos1<tm1.1(cre/ERT2)Zjh> - Head Recombinase Activity Detail MGI Mouse

Nos1^{tm1.1(cre/ERT2)Zjh} - Head
Recombinase Activity Detail

Allele Information | Tissue Information | Images | Recombinase Activity | References

Allele Information

Allele:

Nos1^{tm1.1(cre/ERT2)Zjh}
nitric oxide synthase 1, neuronal; targeted mutation 1.1, Z Josh Huang

Driver: Nos1
Type: Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Synonym:

nNOS-CreER-KI, nNOS-CreER-KI/KO, nNOS-CreERT2-KI, Nos1-CreER-KI, Nos1-CreERT2-KI

Molecular description:

A targeting vector was designed to insert a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the neuronal nitric oxide synthase 1 locus (Nos1). This construct was electroporated into C57BL/6;129S-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with 129S mice to originate the colony. Mutant mice were bred with Actin-FLPe mice to remove the neo selection cassette and the FLPe transgene was subsequently bred out of the line.

Collection:

Neuroscience Blueprint cre

Find mice (IMSR):

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Additional Tissues:

Nos1^{tm1.1(cre/ERT2)Zjh} activity also observed in:
alimentary system, muscle, nervous system, renal & urinary system, sensory organs

Tissue Information

Head

Other recombinase alleles with activity in Head tissues:

7630403G23Rik^{Tg(Th-cre)1Tmd}, Abcg2^{tm1.1(cre/Esr1*)Jvbe}, Acan^{tm1(cre/ERT2)Crm}, Acsm4^tm1(cre)Hsak ...(more)

Images

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Fig. Nos1 Adult Cerebellum

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Fig. Nos1 Adult Cerebral Cortex

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Fig. Nos1 Adult Hippocampus

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Fig. Nos1 Adult Hypothalamus

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Fig. Nos1 Adult Medulla

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Fig. Nos1 Adult Midbrain

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Fig. Nos1 Adult Pons

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Fig. Nos1 Adult Ventral Striatum

J:244340 Fig. 1A

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Recombinase Activity

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Assays Genotypic Background Result Notes

References

All for this allele:

47 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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