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Tg(Kiss1-cre)J2-4Cfe - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Tg(Kiss1-cre)J2-4Cfe
transgene insertion J2-4, Carol F Elias 		Driver: Kiss1
Type: Transgenic (Recombinase)
Synonym: J2-4, Kiss1-Cre
Molecular description: A BAC containing the entire Kiss1 coding region with approximately 109 kb of upstream sequence and 69 kb of downstream sequence (RP24-299B2) was modified by ET-cloning recombineering methods. A DNA fragment encoding cre recombinase followed by the SV40 polyadenylation signal and an Frt-flanked kanamycin resistance marker was inserted at the translational start site of Kiss1. The resistance marker was deleted by arabinose induction of Flp recombinase in bacterial cells during cloning. The purified transgenic construct was injected into one-cell stage embryos from C57BL/6 mice. Three lines (J2-4, J2-4, and J2-6) were generated from the RP24-299B2 construct. J2-4 exhibited cre activity in known areas of Kiss1 mRNA expression and was analyzed in detail.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Tg(Kiss1-cre)J2-4Cfe activity also observed in:
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Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 36 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory