Kiss1<tm1.1(cre/EGFP)Stei> - Nervous System Recombinase Activity Detail MGI Mouse

Kiss1^{tm1.1(cre/EGFP)Stei} - Nervous System
Recombinase Activity Detail

Allele Information | Tissue Information | Images | Recombinase Activity | References

Allele Information

Allele:

Kiss1^{tm1.1(cre/EGFP)Stei}
KiSS-1 metastasis-suppressor; targeted mutation 1.1, Robert A Steiner

Driver: Kiss1
Type: Targeted (Null/knockout, Recombinase, Reporter)

Synonym:

Kiss1^Cre:Gfp, Kiss1-CreGFP, Kiss1^Cre(v1)

Molecular description:

A targeting vector was designed to insert a CreGFP fusion protein cassette and frt-flanked neomycin (neo) selection cassette upstream of the 5' translation start of the KiSS-1 metastasis-suppressor (Kiss1) targeted gene. Specifically, this targeting vector contained an enhanced green fluorescent protein (EGFP) and a Cre recombinase. This construct was electroporated into F1 hybrid (129S6/SvEvTac x C57BL/6NCr)-derived G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females. These Kiss1-CreGFP mice were bred with FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette. When Kiss1 is induced, EGFP immunofluorescence is observed in the hypothalamic arcuate nucleus (ARC) of both sexes and anteroventral periventricular nucleus (AVPV) in females. Homozygotes are often infertile, but heterozygotes display normal fertility.

Find mice (IMSR):

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Additional Tissues:

Kiss1^{tm1.1(cre/EGFP)Stei} activity also observed in:
head

Tissue Information

Nervous System

Other recombinase alleles with activity in Nervous System tissues:

7630403G23Rik^{Tg(Th-cre)1Tmd}, Abcg2^{tm1.1(cre/Esr1*)Jvbe}, Acsm4^tm1(cre)Hsak, Adcyap1r1^{tm1.1(cre/GFP)Rpa} ...(more)

Images

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J:221577 Fig. 6

Recombinase Activity

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Assays Genotypic Background Result Notes

References

All for this allele:

22 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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