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Tg(Krt8-cre/ERT2,-GFP)1Xin - Liver & Biliary System
Recombinase Activity Detail
Allele Information
Allele:
Tg(Krt8-cre/ERT2,-GFP)1Xin
transgene insertion 1, Li Xin 		Driver: Krt8
Type: Transgenic (Inducible, Recombinase, Reporter)
Inducer: tamoxifen
Synonym: K8-CreERT2, Tg(Krt8-cre/ERT2)1Xin
Molecular description: A BAC clone (RP23-234D2) was modified by recombineering methods to insert the cre/ERT2 fusion sequence at the translational start codon of Krt8. An IRES-GFP expression cassette was inserted downstream of the cre sequence. The linearized BAC construct was microinjected into fertilized C57BL/6J oocytes. RT-PCR was used to screen founder mice and one line was chosen for characterization based on its cre/ERT2 expression pattern which closely recapitulated endogenous Krt8 expression. This line carries 2 copies of the transgene (based on quantitative PCR performed on genomic DNA) with a single insertion site. However, although GFP is detectable by RT-PCR and Western blot analysis, GFP fluorescence cannot be visualized by fluorescent microscopy in tissue sections or by FACS analysis of dissociated prostate cells.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Tg(Krt8-cre/ERT2,-GFP)1Xin activity also observed in:
alimentary system, hemolymphoid system, integumental system, renal & urinary system, reproductive system, respiratory system
Tissue Information
Liver & Biliary System Other recombinase alleles with activity in Liver & Biliary System tissues:
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 11 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory