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Ttm1(cre/ERT2)Hssk - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Ttm1(cre/ERT2)Hssk
brachyury, T-box transcription factor T; targeted mutation 1, Hiroshi Sasaki
Driver: T
Type: Targeted (Inducible, Recombinase)
Inducer: tamoxifen
Synonym: TEGFP, TnEGFP-CreERT2
Molecular description: Recombineering of the BAC clone RP23-99E8 containing the T (brachyury) locus generated a targeting vector designed to replace the stop codon of T and introduce sequences encoding the ERAV 2A peptide, a human histone H2B/EGFP fusion protein (protein is expressed in the cell nucleus), the Thosea asigna virus 2A peptide and cre/ERT2 fusion protein. A self-excising ACN cassette (containing the neo resistance marker) was also included. The vector was electroporated into ES cells for homologous recombination. Chimeras were generated from 3 independent clones (36, 41, and 44) and were bred to C57BL/6J females, with the ACN cassette self-excising during passage through the germline. The line derived from #41 was analyzed further.
Find mice (IMSR): Mouse Strains: 0 strains available       Cell Lines: 0 lines available
Additional Tissues: Ttm1(cre/ERT2)Hssk activity also observed in:
embryo-other, mesenchyme, reproductive system
Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Images
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J:194054 Fig. 4
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 9 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/19/2024
MGI 6.24
The Jackson Laboratory