Penk<tm1.1(cre/ERT2)Hze> - Nervous System Recombinase Activity Detail MGI Mouse

Penk^{tm1.1(cre/ERT2)Hze} - Nervous System
Recombinase Activity Detail

Allele Information | Tissue Information | Images | Recombinase Activity | References

Allele Information

Allele:

Penk^{tm1.1(cre/ERT2)Hze}
preproenkephalin; targeted mutation 1.1, Hongkui Zeng

Driver: Penk
Type: Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Synonym:

Penk-2A-CreERT2, Penk-2A-CreERT2-DeltaNeo

Molecular description:

A targeting vector contained a partial Penk intron 1 sequence containing an frt3 site, a partial Penk exon 2 sequence up to (but not including) the endogenous stop codon, a self-cleaving T2A sequence in-frame with the Nxph4 coding sequence, a cre/ERT2 fusion gene, a bovine growth hormone polyA signal, an AttB site, a PGK-Neo-polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, an AttP site and genomic sequence from the Penk locus starting 509 bp downstream of the endogenous stop codon (to create a 511 bp deletion including, the stop codon). This targeting vector was electroporated into G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the AttB/AttP-flanked sequences and replace it with the recombined AttB/AttP site (AttL).

Find mice (IMSR):

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Additional Tissues:

Penk^{tm1.1(cre/ERT2)Hze} activity also observed in:
head

Tissue Information

Nervous System

Other recombinase alleles with activity in Nervous System tissues:

7630403G23Rik^{Tg(Th-cre)1Tmd}, Abcg2^{tm1.1(cre/Esr1*)Jvbe}, Acsm4^tm1(cre)Hsak, Adcyap1r1^{tm1.1(cre/GFP)Rpa} ...(more)

Images

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J:220523 Fig. 5

Recombinase Activity

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Assays Genotypic Background Result Notes

References

All for this allele:

3 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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