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Rasgrf2tm1(cre/folA)Hze - Head
Recombinase Activity Detail
Allele Information
Allele:
Rasgrf2tm1(cre/folA)Hze
RAS protein-specific guanine nucleotide-releasing factor 2; targeted mutation 1, Hongkui Zeng 		Driver: Rasgrf2
Type: Targeted (Inducible, Recombinase)
Inducer: trimethoprim
Synonym: Rasgrf2-2A-dCre, Rasgrf2-2A-DHFR-Cre, Rasgrf2-2A-ecDHFRR12Y/Y100I/Cre, Rasgrf2-2A-ecDHFRR12Y/Y100I/Cre, Rasgrf2-2A-hDHFR/Cre
Molecular description: The targeting vector containied partial Rasgrf2 intron 29-33 sequence containing an frt3 site in intron 33, a partial Rasgrf2 exon 34 sequence up to (but not including) the endogenous stop codon, self-splicing T2A sequence in-frame with the Rasgrf2 coding sequence, a dCre fusion gene in-frame with the Rasgrf2 coding sequence, a bovine growth hormone polyA signal, an AttB site, a PGK-Neo-polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an AttP site. The dCre fusion gene (destabilized Cre, hDHFR/Cre or ecDHFR/Cre) is Cre recombinase fused at its N terminus to the first 159 amino acids of the E. coli K-12 strain chromosomal dihydrofolate reductase gene (DHFR or folA) harboring the G67S mutation and modified to include the R12Y/Y100I destabilizing domain mutations. The targeting vector was electroporated into G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. When treated with trimethoprim, cre reporter gene activity is observed at high levels in cortical layers 2/3 and other scattered cells of the cortex, hypothalamus, thalamus, and midbrain.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Rasgrf2tm1(cre/folA)Hze activity also observed in:
nervous system
Tissue Information
Head Other recombinase alleles with activity in Head tissues:
Images
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J:220523 Fig. 6
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 25 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory