Rorb<tm1.1(cre)Hze> - Head Recombinase Activity Detail MGI Mouse

Rorb^{tm1.1(cre)Hze} - Head
Recombinase Activity Detail

Allele Information | Tissue Information | Images | Recombinase Activity | References

Allele Information

Allele:

Rorb^{tm1.1(cre)Hze}
RAR-related orphan receptor beta; targeted mutation 1.1, Hongkui Zeng

Driver: Rorb
Type: Targeted (Recombinase)

Synonym:

Rorb-IRES2-Cre, Rorb-IRES2-Cre-Delta, Rorb-IRES2-Cre-DeltaNeo/Hygro, Rorb-IRES2-Cre Rorb-IRES2-Cre-DeltaNeo/Hygro, Rorb^{m1(IRES2-Cre)Hze}

Molecular description:

The targeting vector contained, from 5' to 3', a partial Rorb sequence from intron 9 (with an frt3), a partial Rorb exon 10 sequence including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence, a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 late polyA signal, and an AttP site. This construct was electroporated into G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice to remove the AttB/AttP-flanked sequences (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replace it with the recombined AttB/AttP site (AttL). The resulting Rorb-IRES2-Cre-D mice were bred with C57BL/6J wildtype mice to remove PhiC31 transgene.

Find mice (IMSR):

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Additional Tissues:

Rorb^{tm1.1(cre)Hze} activity also observed in:
hemolymphoid system, nervous system

Tissue Information

Head

Other recombinase alleles with activity in Head tissues:

7630403G23Rik^{Tg(Th-cre)1Tmd}, Abcg2^{tm1.1(cre/Esr1*)Jvbe}, Acan^{tm1(cre/ERT2)Crm}, Acsm4^tm1(cre)Hsak ...(more)

Images

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J:220523 Fig. 5

J:220523 Fig. 6

Recombinase Activity

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Assays Genotypic Background Result Notes

References

All for this allele:

28 reference(s)

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