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Myf5tm1(cre/Esr1*)Trdo - Limbs
Recombinase Activity Detail
Allele Information
Allele:
Myf5tm1(cre/Esr1*)Trdo
myogenic factor 5; targeted mutation 1, Thomas A Rando 		Driver: Myf5
Type: Targeted (Inducible, Recombinase)
Inducer: tamoxifen
Synonym: Myf5CreER
Molecular description: A targeting vector was designed to place an internal ribosome entry site (IRES) fused to a CreERTM fusion gene (CreERTM; Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain) downstream of the stop codon in exon 3 of the Myf5 gene. A neomycin resistance marker, flanked by frt sites, was inserted downstream of the IRES-CreERTM cassette. This construct was electroporated into R1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Myf5tm1(cre/Esr1*)Trdo activity also observed in:
embryo-other, head, mesenchyme, muscle
Tissue Information
Limbs Other recombinase alleles with activity in Limbs tissues:
Images
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J:199482 Fig. 2A J:199482 Fig. 2B J:199482 Fig. 2D J:199482 Fig. 3A J:199482 Fig. 3C J:199482 Fig. 4A J:199482 Fig. 4B J:199482 Fig. 4C
J:199482 Fig. S2B J:199482 Fig. S2C
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory