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Tg(Acan-cre/ERT2,-luc)1Gbg - Limbs
Recombinase Activity Detail
Allele Information
Allele:
Tg(Acan-cre/ERT2,-luc)1Gbg
transgene insertion 1, George Bou-Gharios 		Driver: Acan
Type: Transgenic (Inducible, Recombinase, Reporter)
Inducer: tamoxifen
Synonym: Acan-CreER-ires-Luc
Molecular description: A construct was generated where four tandem repeats of the A1 enhancer of the aggrecan gene (-9,477 bp to -9,118 bp of Acan) were cloned upstream of the Col2a1 proximal promoter fragment. The cre/ERT2 sequence, followed by an IRES site and the firefly luciferase cDNA, were inserted after these regulatory elements. The construct was validated by transfection into MEFs isolated from Rosa-lacZ reporter mice, and then used to produce transgenic mice. Founders were crossed with reporter-expressing mice and three showed cre-mediated reporter activity. One line was used for further experiments.
Find mice (IMSR): Mouse Strains: 0 strains available       Cell Lines: 0 lines available
Additional Tissues: Tg(Acan-cre/ERT2,-luc)1Gbg activity also observed in:
Tissue Information
Limbs Other recombinase alleles with activity in Limbs tissues:
Images
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J:208367 Fig. 1 J:208367 Fig. 2
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory