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Tg(Tg-cre/ERT2)1Kero - Respiratory System
Recombinase Activity Detail
Allele Information
Allele:
Tg(Tg-cre/ERT2)1Kero
transgene insertion 1, Jukka Kero 		Driver: Tg
Type: Transgenic (Inducible, Recombinase)
Inducer: tamoxifen
Synonym: TgCreERT2
Molecular description: RedE/RedT-recombination was used to insert a cassette encoding tamoxifen-inducible cre (cre/ERT2), an frt-flanked ampicillin selection marker, and the bovine growth hormone polyadenylation signal at the ATG start codon of the thyroglobulin (Tg) gene in a P1 artificial chromosome (PAC). Following recombination, flp-mediated deletion of the ampicillin marker was performed. The verified construct was linearized, then used for injection of C57BL/6 fertilized oocytes. 5/35 pups tested positive for cre by PCR. Founders were tested for specificity and inducibility of cre recombinase by breeding to lacZ reporter mice and treating double mutants with tamoxifen. 4 founders showed cre activity only in the thyroid gland, and one line with strong and homogenous cre activity was chosen for detailed analyses.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Tg(Tg-cre/ERT2)1Kero activity also observed in:
alimentary system, endocrine system
Images
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J:211398 Fig. 1e third row
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 6 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory