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Tfcp2l1tm1.1(cre/ERT2)Ovi - Alimentary System
Recombinase Activity Detail
Allele Information
Allele:
Tfcp2l1tm1.1(cre/ERT2)Ovi
transcription factor CP2-like 1; targeted mutation 1.1, Catherine E Ovitt
Driver: Tfcp2l1
Type: Targeted (Inducible, Recombinase, Reporter)
Inducer: tamoxifen
Synonym: Tcfcp2l1GCE, Tcf-GCE, Tcftm1(GCE)Ovi, Tfcp2l1tm1(cre/ERT2)Ovi
Molecular description: The targeting construct was generated by inserting the diphtheria toxin A gene (DTA, for positive selection), 3.2 kb 5 homologous arm containing the 5' UTR of exon 1, and 5.5 kb 3 homologous arm containing exon 2 into pBluescript SKII(+). Then the eGFP-CreERT2 (GCE) fragment (McMahon et al. 2008) with an SV40 poly adenylation site and neomycin resistance cassette were inserted immediately downstream of the translational initiation codon ATG. The knock-in construct removed the coding sequences from exon 1 and placed the GCE gene under the control of endogenous regulatory sequences. Flp-mediated recombination removed the selection cassette. The EGFP could not be detected by direct fluorescence or anti-GFP antibody staining.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Tfcp2l1tm1.1(cre/ERT2)Ovi activity also observed in:
head, renal & urinary system, respiratory system, sensory organs
Tissue Information
Alimentary System Other recombinase alleles with activity in Alimentary System tissues:
Images
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J:229520 Fig. 4
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/19/2024
MGI 6.24
The Jackson Laboratory