Tfcp2l1<tm1.1(cre/ERT2)Ovi> - Embryo-other Recombinase Activity Detail MGI Mouse

Tfcp2l1^{tm1.1(cre/ERT2)Ovi} - Embryo-other
Recombinase Activity Detail

Allele Information | Recombinase Activity | References

Allele Information

Allele:

Tfcp2l1^{tm1.1(cre/ERT2)Ovi}
transcription factor CP2-like 1; targeted mutation 1.1, Catherine E Ovitt

Driver: Tfcp2l1
Type: Targeted (Inducible, Recombinase, Reporter)

Inducer:

tamoxifen

Synonym:

Tcfcp2l1^GCE, Tcf-GCE, Tcf^tm1(GCE)Ovi, Tfcp2l1^{tm1(cre/ERT2)Ovi}

Molecular description:

The targeting construct was generated by inserting the diphtheria toxin A gene (DTA, for positive selection), 3.2 kb 5 homologous arm containing the 5' UTR of exon 1, and 5.5 kb 3 homologous arm containing exon 2 into pBluescript SKII(+). Then the eGFP-CreERT2 (GCE) fragment (McMahon et al. 2008) with an SV40 poly adenylation site and neomycin resistance cassette were inserted immediately downstream of the translational initiation codon ATG. The knock-in construct removed the coding sequences from exon 1 and placed the GCE gene under the control of endogenous regulatory sequences. Flp-mediated recombination removed the selection cassette. The EGFP could not be detected by direct fluorescence or anti-GFP antibody staining.

Find mice (IMSR):

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Additional Tissues:

Tfcp2l1^{tm1.1(cre/ERT2)Ovi} activity also observed in:
alimentary system, head, renal & urinary system, respiratory system, sensory organs

Recombinase Activity

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Assays Genotypic Background Result Notes

References

All for this allele:

5 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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