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Tmem119em1(cre/ERT2)Gfng - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Tmem119em1(cre/ERT2)Gfng
transmembrane protein 119; endonuclease-mediated mutation 1, Guoping Feng 		Driver: Tmem119
Type: Endonuclease-mediated (Inducible, Recombinase)
Inducer: tamoxifen
Synonym: Tmem119-CreERT2
Molecular description: The allele was created using CRISPR/cas9 endonuclease-mediated genome editing to target the stop codon of the transmembrane protein 119 locus (Tmem119) on chromosome 5. The cas9-RNP complex and a 2A-CreERT2 dsDNA (encoding a viral 2A oligopeptide that mediates ribosomal skipping and the CreER fusion gene with ~1.5 kbp homology arms were injected into the pronucleus of C57BL/6NTac mouse embryos. Founders were bred to C57BL/6J mice for germline transmission.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Tmem119em1(cre/ERT2)Gfng activity also observed in:
head
Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Images
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J:339857 Fig. 2 J:339857 Fig. 3
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 31 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory